超声介导的"治疗诊断"多功能超分子基因微载体的研究

项目名称： 超声介导的"治疗诊断"多功能超分子基因微载体的研究

项目编号： No.51273177

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 王幽香

作者单位： 浙江大学

项目金额： 80万元

中文摘要： 本课题针对癌症基因治疗的关键问题，将可临床实现的超声微泡技术与非病毒基因载体相结合，制备表面二茂铁修饰的PEG化微泡造影剂，并通过主客体超分子组装，将环糊精修饰的靶向基团及基因纳米粒子组装到微泡造影剂的表面，构筑非病毒基因载体/微泡超分子组装体。利用PEG的长效循环作用和靶向分子的识别作用，实现功能微载体在癌症组织的高效富集；利用微泡的超声成像功能实现对病变组织诊断成像；利用超声"声孔效应"实现非病毒基因载体的多种生物膜跨越传递；利用非病毒基因载体实现基因的高效转染。深入研究这种多功能集成设计方法对延长超声微泡体内循环时间和增强靶向富集功能的影响，探索超声刺激和基因转染协同增强的基本规律，为建立超声辅助癌症基因治疗手段提供切实可行的途径。

中文关键词： 治疗诊断体系；主客体组装；基因载体；微泡；超声

英文摘要： Although the principle of cancer gene therapy has been proved for a long time, there are still many challenges for its clinic application. Armed at the key issue of cancer gene therapy, a new strategy of ultrasound-assisted cancer gene therapy will be explored via the combination of the clinic-realizable ultrasound microbubble technology and the non-viral gene vectors in this research. The multifunctional microcarriers will be specially designed by host-guest assemble of the cyclodextrin-modified gene nanoparticles and targeting ligands onto the ferrocene-modified PEGylated microbubbles. We hypothesize that the multifunctional microcarriers will circulate sufficiently long due to the PEG shell and accumulate in the tumor tissue due to the targeting ligands. Real-time ultrasound diagnostic imaging can be obtained to identify the tumor sites. Microbubble-induced sonoporation can be used as the driving force to transfer gene vectors into cells by improving the permeability of different biofilm, which thus lead to efficient transfection. The synergistic effect of ultrasound stimulus and gene transfection will be explored as a new approach to enhance the in-situ gene transfection. The potential of the novel multifunctional microcarriers in ultrasound-assisted cancer gene therapy will be investigated.

英文关键词： Theranostic system；Host-guest assembly；Gene vector；Microbubble；Ultrasound