调控香雪兰挥发性萜类合成的转录因子克隆及其对萜类合酶基因表达和花香释放的影响

项目名称： 调控香雪兰挥发性萜类合成的转录因子克隆及其对萜类合酶基因表达和花香释放的影响

项目编号： No.31300271

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 高翔

作者单位： 东北师范大学

项目金额： 24万元

中文摘要： 转录因子在植物很多生理和发育过程中发挥着主导作用，挥发性萜类作为次生代谢产物的重要成员，其生物合成同样受多种转录因子调控，其中MYC2和WRKY1被证明可以直接激活倍半萜合酶基因转录，进而控制倍半萜的合成和释放，但是二者如何调控单萜合成还未见报道。 香雪兰花香成分中包含大量的单萜和倍半萜，且各成分之间的释放规律呈现一致性，说明萜类合酶基因的转录调控中可能存在共同的转录因子，但是品种之间单萜和倍半萜的差异很大，暗示可能存在特异性转录因子分别控制两种萜类化合物的合成。 本研究综合利用转录组测序，同源基因克隆，酵母单杂交和挥发代谢谱分析等技术手段，克隆MYC2和WRKY1同源序列并寻找红花和白花香雪兰之间差异表达的转录因子，通过对比它们与萜类合酶基因及代谢产物之间的关系，初步揭示转录因子对香雪兰挥发性萜类物质合成的调控作用。 目前，已经获得多个转录因子和萜类合酶基因序列，为项目实施奠定了基础。

中文关键词： 香雪兰；挥发性萜类物质；转录因子；萜类合酶基因；功能分析

英文摘要： Transcription factors (TFs) play a predominant role in regulating the expression of genes involved in various physiological and developmental processes, including plant secondary metabolism. As an important part of secondary metabolites, volatile terpenoids biosynthesis is also regulated by several TFs, among them, MYC2 and WRKY1 have been proved to activate sesquiterpene synthase gene expression to enhance relevant terpenes biosynthesis and emission. However, their roles in monoterpenes biosynthesis has not been researched. Flowers of Freesia hybrida can emit sorts of monoterpenes and sesquiterpenes in floral scents, and the emission of terpenoid compounds is organized and coordinated. Therefore, it is reasonable to hypothesize that same TFs exist in both two kind of volatile terpenes biosynthesis process. Furthermore, the number of monoterpene and sesquiterpene and their ratio varies between some cultivars which suggests that monoterpene and sesquiterpene biosynthesis is controled by special TFs respectively. In this research, we will utilize transcriptome sequencing, Homologous gene cloning and yeast one-hybrid method to clone MYC2, WRKY1 homologous gene sequence and search differential expression TFs in red and white Freesia hybrida, their expression pattern in different flower development stage, tissues, c

英文关键词： Freesia hybrida；volatile terpene；transcription factor；terpene synthase gene；gene function analysis