玉米幼胚胚性愈伤组织诱导能力全基因组遗传解析

项目名称： 玉米幼胚胚性愈伤组织诱导能力全基因组遗传解析

项目编号： No.31471512

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 沈亚欧

作者单位： 四川农业大学

项目金额： 85万元

中文摘要： 玉米胚性愈伤组织诱导是转基因技术的关键环节，我国高胚性愈伤诱导率骨干自交系匮乏，严重阻碍了转基因育种的快速发展。本项目拟采用高覆盖率重测序构建的玉米IBM连锁图谱及全基因关联分析，深入挖掘与玉米高胚性愈伤诱导率显著相关的SNP标记和QTL位点，验证候选基因功能。首先以包含280份DH系的IBM Syn10群体及其构建的6618个bin marker基因型数据为基础，结合多年多点表型鉴定精细定位高胚性愈伤诱导率QTL区间，发掘控制玉米高胚性愈伤诱导率的关键候选基因。再利用先锋公司开发的高密度SNP芯片对300份玉米自交系进行全基因组关联分析，鉴定与高胚性愈伤诱导率显著相关的SNP位点和关键基因。并利用比较基因组、生物信息学手段，确定并克隆优良等位基因，进而通过转基因和TALEN定点突变验证候选基因功能。结合前期对胚性愈伤诱导过程基因表达调控研究，对玉米胚性愈伤诱导能力进行遗传和调控网络解析。

中文关键词： 玉米幼胚；胚性愈伤组织；诱导能力；遗传解析；全基因组

英文摘要： The induce process of embryonic callus from maize embryo is the core technology in GMO industry. But due to the lack of core inbred lines with high induction efficiency for embryonic callus, the development of maize transgenic breeding was inhibited seriously. In this study, we will combine the high genetic resolution map in IBM population and genome-wide association mapping study, identify and verify the candidate genes with highly significant SNPs and QTLs which related to the trait of high induction efficiency of maize embryonic callus. First of all, we will generate a high density genetics map with 6618 bin markers in a 280 lines IBM Syn10 DH population, with mixed different environment and locations phenotype data to fine mapping the major QTLs. Meanwhile, we are going to use high-resolution SNP microarray for further association study in a 300 hundreds inbred lines population, for the goal of identifying significant SNPs and important candidate genes with the collaboration with DuPont Pioneer. By the method of comparative genomics and bioinformatics, we aim to identify excellent alleles. We also plan to clone these genes from the maize inbred lines with high induction rate of embryonic callus.Then, the functions of the above cloned genes will be validated by both transgenic and TALEN technologies. Combined with previous researches on microRNA regulation and gene expression of embryonic callus induction process, the project will be intended to construct genetic and regulatory networks of induction ability for maize embryonic callus on the whole-genome level.

英文关键词： Maize embryo;Embryonic callus;Induction ability;Genetic analysis;genome-wide