肝病实脾法对免疫性肝损伤小鼠ERS介导肝细胞凋亡影响的研究

项目名称： 肝病实脾法对免疫性肝损伤小鼠ERS介导肝细胞凋亡影响的研究

项目编号： No.81202629

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 郭明章

作者单位： 福建中医药大学

项目金额： 23万元

中文摘要： 《金匮要略》中提到"见肝之病，知肝传脾，当先实脾"的肝病实脾法是中医治疗肝病的重要扶正之法，能提高肝脏疾病的治疗效果。近年来的研究表明在四逆散等疏肝理气方药中加入四君汤等相关健脾方药能有效减少肝脏疾病中肝细胞凋亡减轻肝损伤。肝细胞凋亡导致肝损伤是多种肝脏疾病共有的一种病理变化。细胞内质网应激反应（ERS）介导的肝细胞凋亡是近年发现的重要的肝细胞凋亡途径。本课题建立卡介苗加脂多糖(BCG+LPS)所致小鼠免疫性肝损伤模型，以健脾益气方四君子汤与疏肝理气方四逆散合方，及以四君子汤，四逆散分别做为治疗药，通过对肝功能、肝细胞DNA片段含量检测、肝脏组织病理形态学观察，及ERS介导的细胞凋亡途径相关的CHOP、GRP78、caspase-12的基因和蛋白表达的测定，探讨肝病实脾法对ERS介导肝细胞凋亡的影响机制，对中医治疗肝病之法提高抗肝损伤的疗效机制进行有益的探索。

中文关键词： 实脾法；肝损伤；ERS；细胞凋亡；

英文摘要： Nourishing the spleen is an important treatment to cure to liver disease cure which has been mentioned in 'Synopsis of Prescriptions of the Golden Chamber'.The treatment can reduce the liver cell apoptosis and improve the clinical effect. Recent studies showed that：the prescription of Sinisan soothing Liver to regulate Qi added the prescription of Si Junzi Tang nourishing the spleen and benefiting qi can reduce the liver cell apoptosis and improve the clinical effect.Liver cell apoptosis leading to liver damage is a kind of pathological changes in liver disease. Hepatocyte apoptosis by ERS were found to be important liver cell apoptosis way in recent years. Methods of this study: Using BCG plus LPS to induce immune hepatic injury in mice; Si Junzi Tang and Sinisan, Si Junzi Tang, Sinisan be used as therapeutic medicines; Observing Liver tissue pathomorphologic changes and testing Liver cell DNA fragments content,genes and the protein expression of CHOP,GRP78,caspase-12. Objective: To investigate the influence of nourishing the spleen on the hepatocyte apoptosis by ERS of immunized mouse liver damage,and explore the righting method of TCM treatment applied in liver disease.

英文关键词： Nourishing the spleen；Liver injury；ERS；apoptosis；