磷脂对三疣梭子蟹卵黄发生的营养调控机制研究

项目名称： 磷脂对三疣梭子蟹卵黄发生的营养调控机制研究

项目编号： No.41476125

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 天文学、地球科学

项目作者： 周歧存

作者单位： 宁波大学

项目金额： 90万元

中文摘要： 迄今为止，有关磷脂对三疣梭子蟹卵巢发育与卵黄发生的营养调控机制研究尚未见报道。本项目运用现代营养学、生物化学、生理学和分子生物学等方法，研究三疣梭子蟹雌蟹卵巢发育及卵黄发生过程中磷脂的消化、吸收、转运、合成和代谢过程。具体的研究内容如下：（1）研究不同磷脂源及水平对三疣梭子蟹生长、肝胰腺及卵巢磷脂代谢关键酶活力及基因表达的影响，阐明三疣梭子蟹卵巢发育不同阶段磷脂合成、转运及代谢的差异；（2）研究磷脂对三疣梭子蟹血淋巴、肝胰腺及卵巢内分泌激素的影响，揭示磷脂对三疣梭子蟹内分泌激素调控的生理和分子机制；（3）通过关闭或开放某些特定关键控制点，探明三疣梭子蟹卵黄发生与卵黄蛋白源形成的营养调控机制；（4）通过营养干预，探讨磷脂通过内分泌系统和相关分子调控卵黄的合成。预期研究成果不仅可以丰富三疣梭子蟹生殖调控和分子营养学的基础理论，而且对于三疣梭子蟹的资源增殖和保护具有重要意义。

中文关键词： 三疣梭子蟹；磷脂；卵黄发生；营养代谢；调控机制

英文摘要： Up to now, the nutritional regulation mechnisms of dietary phospholipids on vitellogenesis for swimming crab have not been reported. In the present project, modern nutrition, biochemistry, physiology and molecular biology are used to demonstrate the key factors of nutritional regulation on vitellogenesis of swimming crab. Specific research contents are as follows: (1) to study the effect of dietary phospholipids sources and levels on growth performance, ovarian development and vitellogenesis, it will clarify phospholipids digestion, absorption, transport, synthesis and metabolic processes during ovarian development and e vitellogenesis for swimming crab; (2) to study the effect of dietary phospholipids on hemolymph, liver-pancreas and ovarian endocrine hormones, it reveals endocrine physiology and molecular mechanisms of phospholipids on hormonal regulation; (3)by closed or open some specific critical control points, it will prove nutritional regulation mechanism on vitellogenesis and formation of yolk protein source; (4) by nutritional intervention, it will explore yolk protein synthesis by phospholipids through the endocrine system and related molecules regulation. The results of present study can not only enrich the basic theory of reproductive control and molecular nutrition, but also it will play important role in resource protection and proliferation for swimming crab.

英文关键词： Swimming crab;Phospholipids;Vitellogenesis;Nutritional metabolism;Regulatory mechanism