藏药绿萝花中作用于2型糖尿病PTP-1B，PPARs多靶标的活性成分及作用机制研究

项目名称： 藏药绿萝花中作用于2型糖尿病PTP-1B，PPARs多靶标的活性成分及作用机制研究

项目编号： No.81460650

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李敏

作者单位： 江西中医药大学

项目金额： 47万元

中文摘要： 2型糖尿病是一种胰岛素相对缺乏或胰岛素抵抗导致的以血糖增高为特征的慢性疾病，危害巨大，治愈难，寻找高效低毒的抗2型糖尿病药物刻不容缓。藏药绿萝花为瑞香科结香属植物滇结香的干燥花蕾。本课题组前期利用2型糖尿病模型细胞和PPAR-γ因子激活实验确定了绿萝花的有效部位PE及EA，并于EA部位中分离得到12个单体化合物。本项目拟采用多种色谱、光谱学技术及体外PPAR-γ因子激活实验活性跟踪法对绿萝花有效部位进行成分分离鉴定；借助HepG2,3T3-L1胰岛素抵抗细胞模型，利用Western blot研究化学成分/群对抗2型糖尿病靶点基因PTP-1B，PPAR-γ/α表达的影响；并通过Caco-2单层细胞模型预测各体外活性成分的吸收情况；利用基因技术研究活性成分对2型糖尿病及其并发症多重基因表达谱的影响，最终揭示其作用机制，明确物质基础，为新的抗2型糖尿病药物的发现及绿萝花的深入研究提供实验室依据。

中文关键词： 藏药绿萝花；2型糖尿病；多靶标；活性成分；作用机制

英文摘要： Type 2 diabetes is a chronic disease characterized by hyperglycemia which is resulted by the lack of insulin or insulin resistance, its harm is huge and difficult to cure, so we should look for drugs with efficiency and low toxicity against type 2 diabetes.In our previous research,Edgeworthia gardneri Meissn showed significant activity against type 2 diabetes,we use type 2 diabetes model cells and PPAR-γ activating factor experiments to determine the effective part of the PE and green dill flower EA, and in EA part 12 compounds were isolated . The project intends to adopt a variety of chromatographic , spectroscopic techniques and in vitro activation of PPAR-γ factor experimental activity tracking method for the effective parts of green dill flower composition isolated and identified ; With HepG2, 3T3-L1 cell model of insulin resistance , the use of Western blot study chemical composition / group against the impact of type 2 diabetes target gene PTP-1B, PPAR-γ / α expression ; through Caco-2 cell monolayers in vitro model to predict the absorption of each active ingredient ; using gene technology research active ingredient and type 2 diabetes multiple complications affect gene expression, and ultimately reveal its mechanism of action, clear material basis , provide laboratory evidence for the discovery of new drug resistant type 2 diabetes and in-depth research and development of Edgeworthia gardneri Meissn.

英文关键词： Edgeworthia gardneri;2-DM;targets;constitutes;mechanism