一种用于生物分子多重检测的微加工图形编码悬浮芯片阵列

项目名称： 一种用于生物分子多重检测的微加工图形编码悬浮芯片阵列

项目编号： No.31300817

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 郑克孝

作者单位： 中国科学院苏州纳米技术与纳米仿生研究所

项目金额： 23万元

中文摘要： 生物信号检测技术一直在向样品和分析物的大规模并行处理方向发展。然而，在以微孔板为代表的样本高通量产品和以微阵列、测序技术为代表的分析物高通量产品不断推进这两方面分析能力提升的同时，人们发现缺乏可靠的技术来应对组学研究、生物标识物验证、分子诊断、药物筛选等领域中对大量样品进行10-1000重并行检测的需求。悬浮阵列技术使用编码微珠作为检测载体，具有多重性、灵敏度、可靠性、扩展性等诸多优势，显示出解决"在单个微量反应体系内并行检测多个指标"问题的潜力。但现有各种方案在材料、结构、制备、仪器及解码方式等方面尚存在不足之处。本项目提出利用高反射介电镀层作为编码物理结构、通过镀层和基底间光学透射率的差异对悬浮芯片进行二维图形编码的方案。该芯片只含无机非金属材料，可采用成熟的微加工技术大量制造，结构简单、成本低廉、易于修饰，具有优异的可重复性。作为原理性验证，我们将用其检测1型糖尿病的多重生物标识物。

中文关键词： 悬浮芯片；多重检测；图形编码；生物标识物；微加工

英文摘要： Massive parallel processing of samples and analytes stands long as a major goal of technology development for biological signal detection.However, whilst the limits of these two dimensions are constantly pushed forward by "sample high-throughput" products like microplates and by "analyte high-throughput" products like microarrays and sequencing, researchers now find themselves being short of reliable tools for assaying substantial amount of samples with 10-1000 plex indices per sample; which coincidentally manifests the urgent need for efficient quantitative assays from multiple disciplines such as omics study, biomarker validation, molecular diagnostics, and drug screening, etc. Using analyte-addressable, physically-coded microbeads as assay units, suspension arrays show great potential to accomplish the quest of parallel detection of multiple indices within a single micro reaction volume, due to their superior performances in multiplexity, sensitivity, reliability, and scalability. Yet current solutions still bear defects in material, structure, fabrication, instrumentation or decoding methods.This application proposes a novel scheme using the optical transmittance pattern created by high-reflection dielectric coating as a 2-D graphical code for suspension array beads. Mass-produced via well-established micro

英文关键词： suspension array；multiplex assay；graphical encoding；biomarkers；microfabrication