海藻酸钠-玉米醇溶蛋白/纳米羟基磷灰石复合支架的仿生构建和性能研究

项目名称： 海藻酸钠-玉米醇溶蛋白/纳米羟基磷灰石复合支架的仿生构建和性能研究

项目编号： No.51303006

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 张彩云

作者单位： 安徽中医药大学

项目金额： 19万元

中文摘要： 纳米羟基磷灰石/高分子仿骨支架材料的研究取得了显著进展，但材料的力学性能和生物学性能仍难满足临床需求。针对如何提高海藻酸钠凝胶结构稳定性和生物学性能，本项目联合凝胶复合和仿生矿化两种手段制备海藻酸钠-玉米醇溶蛋白/纳米羟基磷灰石(SA-Zein/HA)仿骨支架材料。首先基于蛋白质-多糖相互作用，利用玉米醇溶蛋白改善海藻酸钠凝胶的表面和力学性能并增加矿化位点；然后对复合凝胶进行仿生矿化诱导羟基磷灰石在凝胶体系中均匀生长和有序排列，最后通过冷冻干燥技术构建SA-Zein/HA多孔支架。研究复合凝胶体系的组成、结构对性能的影响，结合矿化条件探讨羟基磷灰石晶体在复合凝胶中的生长机制；通过力学性能、表面性能和降解性能等测试优化材料制备工艺；考察细胞在支架上的生长增殖情况，评价材料的生物相容性，为SA-Zein/HA多孔支架最终应用于组织工程仿骨支架及新型轻质高强骨修复材料的设计提供科学实验依据。

中文关键词： 凝胶；仿生矿化；海藻酸钠；玉米醇溶蛋白；羟基磷灰石

英文摘要： In recent years, researches on hydroxyapatite/biopolymers bone scaffolds have made significant progress, but their mechanical properties and biological performance are still difficult to meet clinical needs. To improve the structural stability and biological properties of sodium alginate gel, gel modified and bio-mineralization are employed to prepare sodium alginate -Zein/nano-hydroxyapatite (SA-Zein/HA) porous bone scaffold. Firstly, zein will be used to improve the surface property and mechanical property of sodium alginate gel based on interactions between protein and polysaccharide, at the same time the active groups of zein will increase nucleation sites during the biomineralization of HA. Secondly, HA crystals are biomineralized in SA-Zein composite gel with evenly dispersion and ordered arrangement. At last, SA-Zein/HA porous scaffolds with high mechanical property and biological performance will be fabricated through the thermal induced phase separation and freeze drying technique. The growth mechanism of nano-hydroxyapatite crystal in this gel system will be studied from the influence of gel composition, spatial structure and the biomineralization conditions on hydroxyapatite nucleation, growth and arrangement. Material formula will be optimized by the test of mechanical properties, surface properties

英文关键词： gel；bio-mineralization；sodium alginate；zein；hydroxyapatite