茯茶中冠突散囊菌作用下的代谢物变化及形成机理研究

项目名称： 茯茶中冠突散囊菌作用下的代谢物变化及形成机理研究

项目编号： No.31471706

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 刘仲华

作者单位： 湖南农业大学

项目金额： 84万元

中文摘要： 冠突散囊菌是茯茶加工过程中的优势微生物，是茯茶品质形成的主要生化动力来源，但关于茯茶中冠突散囊菌作用下的代谢物变化及形成的研究较少，严重阻碍了茯茶品质风味形成机理及功能活性成分的深入研究。本项目拟采用项目组前期分离的茯茶优势微生物-冠突散囊菌为单一发酵菌株，以无菌黑毛茶为底物进行固态发酵。采用HPLC-MS/MS和HPLC-NMR联用技术分析发酵前后茶叶化学物质组成差异；根据分子质量信息和光谱信息结合标准品、数据库、文献等对差异代谢物进行鉴定，并筛选出由儿茶素/茶氨酸/生物碱衍生形成的特征代谢物；通过儿茶素/茶氨酸/生物碱不同组合为底物进行液态发酵，明确特征代谢物的底物来源及生物合成途径；通过蛋白质组学技术研究催化茯茶中代谢物变化与特征代谢物形成的微生物酶；以期探明茯茶中冠突散囊菌作用下的代谢物变化及形成机理，为揭示茯茶品质风味形成机理及茯茶深度开发利用奠定理论基础。

中文关键词： 茯茶；冠突散囊菌；代谢物；微生物酶；形成机理

英文摘要： Eurotium cristatum is the dominating microorganism during the processing of Fuzhuan tea. It is also the source of catalyzing enzyme for the quality characteristic of Fuzhuan tea. However, there are few studies on the change and formation of metabolites in Fuzhuan tea during fermentation by Eurotium cristatum, which significantly restrict the development of research on the mechanism for the formation of the quality characteristic and bioactive components of Fuzhuan tea. In this project, Eurotium cristatum are used as single strain culture and sterile raw dark green tea are used as substrate for the solid state fermentation. HPLC-MS/MS and HPLC-NMR are used for analyzing the difference of chemical constitution between the samples before and after fermentation. According to the molecular weight and spectrographic information of metabolites, standards, database and reference, the difference metabolites will be identified and the characteristic metabolites, which derived from catechin, theanine and alkaloid, are selected. The origin of substrate and the biosynthetic pathway of characteristic metabolites are clarified by liquid state fermentation, which used various combinations of catechin, theanine and alkaloid as substrate. The enzyme catalyzing the change of metabolites and the biosynthesis of characteristic metabolites are studied by the proteomic technology. This objective of this project is revealing the mechanism for the change and formation of metabolites in Fuzhuan tea during fermentation by Eurotium cristatum and is providing a theoretical basis for the researchs on the mechanism for the formation of the quality characteristic and comprehensive exploitation of Fuzhuan tea.

英文关键词： Fuzhuan tea;Eurotium cristatum;metabolite;microbial enzymes;mechanism