肿瘤生长初期基质层生理特性的活体多光子显微成像研究

项目名称： 肿瘤生长初期基质层生理特性的活体多光子显微成像研究

项目编号： No.81201123

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 郑炜

作者单位： 中国科学院深圳先进技术研究院

项目金额： 23万元

中文摘要： 了解肿瘤发展早期基底组织的生理特性对癌症的诊断和治疗具有非常重要的意义。但是由于受到探测技术的限制，对基底组织的活体显微成像研究始终不足。基底组织内胶原纤维（collagen）和基质层细胞（stromal cell）的生理特性随肿瘤发展的变化机制至今还不是很清楚。为此，本项目拟从（1）开发高稳定、高分辨、高重复、多光子显微成像技术；（2）创建合适的裸鼠背部皮窗肿瘤模型；（3）探索高效的数字显微图像特征信息提取方法三个方面入手，活体、原位、动态地研究基底组织内胶原纤维和基质层细胞在早期肿瘤发展过程中的形态、位置、数量等特性随时间的变化情况，从而揭示肿瘤基质层生物自发光学信号（包括自发荧光和二次谐波的图像、光谱信号）与肿瘤发展各阶段之间的关联特性，为进一步实现癌症的早期临床光学诊断提供科学研究基础.

中文关键词： 双光子显微成像；自体荧光；二次谐波；小鼠背部皮窗；肿瘤

英文摘要： Achieving more knowledge about the morphological and physiological characteristics of stroma in precancer will certainly increase our understanding of the mechanism of cancer progression and, as a result, improve the outcome of therapy. However, due to the limitation of technique, few microscopy studies have been carried out to investigate the dynamic changes of stroma in vivo. The mechanism of tumor-induced changes on both collagen fiber and stromal cells in the stroma is unclear. This proposal provides a new strategy to study the dynamic changes of morphological and physiological characteristics of the stroma in precancer development in vivo. An animal tumor model based on the technique of dorsal skinfold chamber in nude mouse will be used as the experiment sample and a home-made high-performance multiphoton microscopy system will be employed to measure both second harmonic generation (SHG) and two-photon excitation autofluorescence signals from the stroma. The time-lapse nonlinear optical images from the same target during cancer progression will be taken and then analyzed with quantitative digital image process methods. Meanwhile, other intrinsic optical signals such as the fluorescence spectra and the SHG anisotropy will also be studied. The purpose of this study is to explore the relationship between the i

英文关键词： two-photon microscopy；autofluorescence；second harmonic generation；mouse skinfoulder chamber；tumor