番茄FAF基因的图位克隆及其调控果实发育的机理研究

项目名称： 番茄FAF基因的图位克隆及其调控果实发育的机理研究

项目编号： No.31501773

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 徐倩

作者单位： 中国科学院上海生命科学研究院

项目金额： 21万元

中文摘要： 重要蔬菜作物番茄是研究果实生长发育的模式植物，其果实的大小和形状等外观特征是重要的品质性状。在番茄果实形成和生长发育过程中，由于遗传或栽培等因素会产生裂果和畸形果，不仅影响其外观品质，也影响营养品质的形成。以往研究多关注果实大小和果型等并在分子基础上取得了较大进展，而畸形果形成的分子机理研究较少。本项目拟以EMS诱变筛选到的一个果实外观异常的faf突变体为研究材料，通过对FAF基因的图位克隆，并应用细胞学、遗传学、基因表达分析以及组学等方法手段，阐明FAF基因参与心皮发育的分子调控机理。研究结果将有助于完善果实形成的分子调控机理，并为番茄等作物的果实品质改良提供理论依据。

中文关键词： 番茄；器官形成；基因功能；数量性状位点；果实

英文摘要： Tomato is an important vegetable crop and an excellent model for fruit developmental study. The appearance of tomato fruit including size and shape as well as smoothness is an important quality character. Due to genetic defects and suboptimal growth conditions, cracked and/or malformed tomato fruits are produced, causing yield and quality loss. In the past, great attention has been paid to dissection of the genetic and molecular basis underlying fruit size and shape in tomato. Little is known about the molecular mechanism underlying the regulation of fruit malformation. We identified a mutant from our EMS mutagenized tomato population, fasciated flower and fruit (faf), showing defective flower and fruit development. In this proposal, we plan to map-based clone the FAF gene and study its functions in fruit growth and development through multiple approaches, including phenotyping, histological analysis and gene expression. The results obtained from the project will further our understanding on the molecular mechanism underlying whereby FAF regulates fruit formation and provide strategies for improvement of tomato and other fruit-bearing crops.

英文关键词： Tomato;Organogenesis;Gene function;Quantitative trait loci;fruit