马尾松高抗旱家系应答干旱胁迫的分子机理

项目名称： 马尾松高抗旱家系应答干旱胁迫的分子机理

项目编号： No.31260183

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 丁贵杰

作者单位： 贵州大学

项目金额： 50万元

中文摘要： 马尾松是我国最主要工业用材树种之一。项目拟采用自己选育的既速生丰产，又对干旱胁迫高抗的种质资源作为研究材料，利用转录组文库的高通量测序与cDNA Microarray技术相结合策略，获得干旱胁迫与正常水分下的差异表达基因，克隆到差异表达的EST（Expressed Sequence Tag），根据其功能筛选可能的抗旱相关EST；揭示马尾松在干旱胁迫下抗旱相关EST的时空表达，结合相关酶活性变化，建立干旱胁迫下抗旱基因的表达谱，在转录水平上解析马尾松应答干旱胁迫机制；最终筛选和克隆在抗旱中起重要作用的抗旱相关基因的全长序列。项目开展，不仅在转录水平解析马尾松高干旱种质抗旱特性的分子机制，也将为抗旱相关基因克隆和新的马尾松抗旱资源的筛选， 以及通过遗传工程创造抗旱新种质奠定基础；同时，项目也能直接为合理利用这一资源提供新思路，为全面准确的应用该良种打下坚实的理论基础。

中文关键词： 马尾松；抗旱家系；分子机理；基因表达；酶活性

英文摘要： Pinus massoniana is the most important conifer industrial timber in China, however, the drought severely prohibited the fast-growing and high-yield of this tree. Fortunately, we had obtained an elite genotype which showed significantly higher tolerance to drought. To unravel the molecular mechanism to this stress, the present program will focus on distinguishing the differentially expressed genes under drought stress using the strategy of next-generation sequencing and cDNA microarray technology, followed by the temporal and spatial expression profile elucidation. Consequently, the chief candidate genes closely related to drought stress of pine will be dug up, and the full length of drought-related genes may be cloned. Therefore, the ongoing project may elucidate the molecular mechanism underlying the elevated tolerance to drought stress in this germplasm, the molecular information and cloned genes can also provide a substantial platform for the genetic breeding for drought tolerance in this tree, as well as for further utility of this elite germplasm.

英文关键词： Pinus massoniana；Resistant drought family；Molecular mechanisms；Gene expression；enzyme activity