皮层下促觉醒脑区支配的内侧前额叶皮层神经回路活动及处理信息的特征研究

项目名称： 皮层下促觉醒脑区支配的内侧前额叶皮层神经回路活动及处理信息的特征研究

项目编号： No.31271165

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 夏建霞

作者单位： 中国人民解放军第三军医大学

项目金额： 75万元

中文摘要： 内侧前额叶皮层是与脑认知功能和觉醒水平高度相关的脑区，接受大量皮层下觉醒系统纤维的兴奋性输入，是觉醒期海马短期记忆的主要信息来源。最新研究表明，内侧前额叶皮层神经回路与海马神经回路间振荡活动的偶联是学习和记忆形成的功能基础。在前期研究外侧下丘脑促醒肽orexins对前额叶皮层神经元活动影响的基础上，本研究拟采用在体电生理、光遗传学、神经药理学、微透析、免疫组化技术和分子生物学技术，在体观察睡眠觉醒周期中，皮层下促觉醒脑区支配的内侧前额叶皮层神经元所构成的神经回路在睡眠觉醒周期中的活动特征，以及与信息处理的相关性；觉醒系统不同活动水平下，该神经回路活动的变化以及信息处理和学习记忆行为的改变。以期通过对觉醒系统支配的内侧前额叶皮层神经回路，参与信息获得、编码、短时储存及提取等觉醒相关的学习记忆活动关系的阐述，为理解大脑皮层认知功能的神经生物学基础以及持续觉醒时认知功能受损的治疗提供理论依据。

中文关键词： 前额叶皮层；觉醒；学习记忆；神经回路；

英文摘要： The prefrontal cortex (PFC), especially the medial PFC (mPFC), receiving abundant projections from subcortical arousal systems, is the most evolved brain regions highly associated with the level of wakefulness and plays a critical role in many higher cognitive functions, such as working memory and attention. A line of evidence support the notion that in concert with hippoampus, the mPFC is the major input source of hippocmapus in the establishement of short-term memory in wakfulness. Specially, the coupled communication of neural circuits between mPFC and hippocampus may be the key for working memory performance. Our previous results have demonstrated that application of arousal-promoting neuropeptide orexins could excite the mPFC neurons in vitro, while little is known about the characteristics and significance of arousal systems-innervating neural circuits in mPFC across sleep and wakefulness states in vivo. In this project, we will first investigate the characteristics of these neural circuits dominated by subcortical arousal systems, especially, orexinergic and noradrenergic afferents in sleep and wakefulness cycle. Then the activity of these neural circuits and different process of visual information as well as that of working memory performance will be recorded. And the relationship between activity of t

英文关键词： prefrontal cortex；wakefulness；learning and memory；neural circuit；