中药五谷虫对创面愈合相关因子分泌和修复细胞凋亡基因表达的影响

项目名称： 中药五谷虫对创面愈合相关因子分泌和修复细胞凋亡基因表达的影响

项目编号： No.30873336

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 吕德成

作者单位： 大连医科大学

项目金额： 31万元

中文摘要： 蛆虫，中医学称之为五谷虫，为丽蝇科丝光绿蝇或其它近缘昆虫的幼虫。五谷虫疗法，是近年来兴起的有效治疗耐药菌引起的严重感染创面的新型生物治疗方法。临床上应用活体五谷虫来治疗耐药菌感染创面，已取得满意效果，其作用机制主要包括：生物清创、抗菌和促进创面愈合。本课题通过对医疗用五谷虫的制备技术研究，获得安全、无菌的活体五谷虫和粉剂；应用药物化学提纯方法，提取出五谷虫干粉中的脂肪酸成分。结果发现：（1）活体五谷虫匀浆产物及中药五谷虫干粉作用于创面，可减少大鼠皮肤创面的愈合时间、提高创面的愈合率和愈合质量。（2）体外实验发现，活体五谷虫分泌物（ES）通过激活PI3K/AKT1通路，对人微血管内HMEC-1的迁移具有促进作用。（3）活体五谷虫的匀浆物可促进大鼠皮肤创面P物质（SP）的分泌和蛋基因产物 9.5（PGP9.5）的表达，进而促进大鼠皮肤创面神经再生。（4）五谷虫干粉中的脂肪酸成分可通过促进大鼠皮肤创面血管生成来促进创面愈合。（5）该脂肪酸提取物可在创面愈合早期抑制创面修复细胞凋亡来促进创面愈合，晚期通过促进创面修复细胞凋亡来抑制瘢痕生成。

中文关键词： 五谷虫；创面愈合；VEGFA；细胞凋亡

英文摘要： Traditional Chinese medicine "WuGuChong" is the larvae of Lucilia sericata, Chrysomyia megacephala or other relative Calliphoridae insects. Nowadays, WuGuChong therapy, as a kind of biological therapy, has been widely used to treat various refractory wounds including diabetes foot and venous ulcers. The therapeutic action contains biological debridement, disinfection and enhancement of wound healing. In this study, we prepared aseptic living WuGuChong homogenate products, dried WuGuChong and fatty acid extracts from dried WuGuChong. We studied the Effect of the above substance on wound healing and related mechanisms. The result demonstrated that both living WuGuChong homogenate and dried WuGuChong can reduce the rat skin wound healing course and improve wound healing rate and quality of healing. WuGuChong excretion/secretion (ES) significantly induced HMEC-1 cell migration in both wound healing and transwell assays, and this event is partially mediated by the activation of AKT1. WuGuChong homogenate up-regulates SP and protein gene product 9.5 expressions during wound healing process, thereby promoting neural regeneration and wound healing. Fatty acid extracts from dried WuGuChong can promote murine cutaneous wound healing probably resulting from the powerful angiogenic activity of the extracts. Fatty acid extracts could inhibit apoptosis of wound repair cells in prophase thereby promoting wound healing, and promote apoptosis of wound repair cells in advanced stage in order to inhibit scar formation.

英文关键词： WuGuChong;wound healing;VEGFA;apoptosis