基于RNA-Seq的沙漠植物花花柴高温胁迫响应的分子机理研究

项目名称： 基于RNA-Seq的沙漠植物花花柴高温胁迫响应的分子机理研究

项目编号： No.31460071

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 王彦芹

作者单位： 塔里木大学

项目金额： 50万元

中文摘要： 随着全球气候变化，极端高温对植物发育及作物产量造成的影响越来越受到人们的关注，植物耐高温的分子机制已成为植物抗非生物逆境研究的重要方向。本项目在前期研究中发现：生长在塔里木盆地的沙漠植物花花柴，可以在45℃以上条件下正常开花结实，是宝贵的耐高温天然资源。本项目拟以花花柴为材料，通过细胞学、生理生化分析探讨其耐高温的生理基础；并结合RNA-seq转录组测序及分析等系统研究，探讨花花柴响应高温胁迫的代谢与信号网络变化，确定几个高温胁迫响应的关键候选基因。通过构建关键候选基因的超表达和RNAi载体，并转化拟南芥和花花柴研究基因的功能。研究结果将揭示花花柴耐高温的生理与分子基础，同时为耐高温作物的培育提供理论基础和基因资源。

中文关键词： 沙漠植物；花花柴；高温胁迫；RNA-Seq

英文摘要： With the temperature change of global, heat stress is becoming an important limiting factor for plant development and crop yields. Thus, the molecular machanism of plant tolerance to heat stress is become an important research task in abiosis stress. Our preliminary data showed that eremophyte Karelinia caspia L.，is able to bloom and fructify normally above 45℃. It was regarded as a valuable plant natural resources for research tolerance to heat stress. In this study, we will research cytology and physiology basic systematically through observing the micro-structure and analyzing physiological and biochemical. Integrating the result of transcroptome to speculate metabolic and signal networks of responce to heat stress in Karelinia caspia. Some key functional cadidate genes would be verified for response to high temperature stress. Lastly, cloning ORF of these genes for tolerance to heat stress,constructing over-expression vector and RNAi vector, transforming into Arabidopsis thaliana and Karelinia caspia.The characteristic of tolerance to high temperature will be studied in transgene plants. Through the proposed research, it would be revealed the physiological and molecular mechanisms in eremophyte Karelinia caspia, as well as to providing fundmental basis and gene resource for crop breeding for tolerance to heat stress.

英文关键词： eremophyte;Karelinia caspia;high temperature stress;RNA-Seq