双酚A影响稀有鮈鲫卵巢发育的生态毒理基因组学研究

项目名称： 双酚A影响稀有鮈鲫卵巢发育的生态毒理基因组学研究

项目编号： No.31270547

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 王在照

作者单位： 西北农林科技大学

项目金额： 81万元

中文摘要： 双酚A(BPA)是一种广泛应用化学品，但是它具有内分泌干扰作用，目前它的生殖毒性备受关注，本项目在预实验的基础上使用对BPA反应敏感的稀有鮈鲫为实验动物，通过分析卵巢的发育状况，检测性类固醇激素和卵黄蛋白原水平，用转录组测序和实时定量PCR的基因组学技术，研究低剂量和高剂量BPA对卵巢发育的影响，阐明低剂量BPA抑制卵巢发育的毒理途径,探索高剂量BPA对卵巢发育的影响及其分子机制，获得BPA早期预警的敏感分子生物标志物，探索 BPA非单调剂量效应的分子机制。本项目的研究成果将有助于对BPA进行正确的生态风险评价，也有助于获得稀有鮈鲫在BPA毒性作用下的特征性整体表达图谱，增加BPA生态风险评价的确定性，获得稀有鮈鲫大量的基因组序列，为其生态毒理基因组学的广泛应用奠定基础。

中文关键词： 稀有鮈鲫；双酚A；转录组；生态毒理；性腺

英文摘要： Bisphenol A (BPA) has wide industrial applications and was testified to be an endocrine disrupting chemical. Its reproductive toxicity has been paid increasing attentions. Based on our previous findings, the present study will investigate the impacts of low-dose and high-dose BPA on the ovarian development in rare minnow Gobiocypris rarus, which was confirmed to be sensitive to BPA, by histological analysis of ovarian development, sex steroids assay, vitellogenin measurement, the genomic methods of high-through RNA sequencing technology and real-time PCR analysis. The findings of present study will elucidate the toxicological pathways of inhibiting effects on ovarian development for low-dose BPA, explore the effects of high-dose BPA on the ovarian development and its molecular mechanisms, provide sensitive molecular biomarkers for BPA early warning, and explore the molecular mechanism of nonmonotonic effects in BPA exposure. The present study will facilitate exact ecological risk assessments of BPA, provide a whole characteristic gene expression profile in rare minnow for BPA exposure, increase the certainty for BPA ecological risk assessments, and produce high-through gene sequence data which would be significant to extend ecotoxicogenomics studies in rare minnow.

英文关键词： Gobiocypris rarus；bisphenol A；transcriptome；ecotoxicology；gonads