玉米耐渍候选基因zmERF33的功能分析

项目名称： 玉米耐渍候选基因zmERF33的功能分析

项目编号： No.31271741

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 杜何为

作者单位： 长江大学

项目金额： 80万元

中文摘要： 在已获得194个玉米AP2/ERF家族基因的基础上，分析了ERF亚族B2亚组20个基因在玉米自交系Hz32（耐渍系）和Mo17（敏感系）根系中的表达，发掘并克隆了1个玉米耐渍候选基因zmERF33。CaMV35s启动子驱动zmERF33基因导入拟南芥的阳性转化体，其耐渍性显著改良。本研究拟将耐渍候选基因 zmERF33、35S::△2 zmERF33遗传转化导入玉米自交系Mo17，反义 zmERF33、35S::HA::zmERF33基因导入玉米自交系Hz32，详细分析转化体的植株表型特别是根系形态、组织结构、生理生化、激素水平等的变化，以及这些性状在淹水胁迫下的变化；探索zmERF33基因所参与的表达调控网络；阐明该基因的生物学功能、与玉米耐渍性的关系及其调控玉米耐渍性的分子机制。该研究对阐明玉米耐渍性的生物学基础积累有益的资料，为利用zmERF33基因创制玉米耐渍新种质提供理论支撑。

中文关键词： 玉米；非生物胁迫；涝渍胁迫；转录因子；基因克隆

英文摘要： A total of 194 genes of APETALA2/Ethylene Response Factor (AP2/ERF) family were identified in maize. Recent research has shown that genes in B2 group of ERF subfamily can enhance plant responses to waterlogging stress or hypoxia. This study studied twenty genes from B2 group of maize ERF subfamily. Their expression profiles in the roots of maize inbred line Hz32 (waterlogging tolerance line) and Mo17 (waterlogging sensitive line) were analyzed, respectively. Results show that zmERF33 is a candidate gene for waterlogging tolerance. Transgenic Arabidopsis plants, which over-expressed zmERF33, exhibited an improved tolerance of waterlogging. In this study, 35S::zmERF33 and 35S::△2 zmERF33 will be transferred into Mo17, respectively. Antisense 35S::zmERF33 and 35S::HA::zmERF33 will also be transferred into Hz32. For the transgenic plants above, a number of traits will be investigated, including root morphology, tissue structure, enzyme activity on fermentation pathway, and hormones levels, both in normal condition and under waterlogging stress. Efforts are made to explore the network and biological functions of zmERF33 gene. Focus has been put on explaining the molecular mechanism of waterlogging tolerance in maize. The functional analysis of zmERF33 gene will be of benefit for understanding maize responses to water

英文关键词： Maize (Zea mays L.)；Abiotic stress；Waterlogging stress；Transcription factor；Gene cloning