下颌髁突软骨干细胞的特性及其促进骨关节炎状态下关节软骨修复再生的研究

项目名称： 下颌髁突软骨干细胞的特性及其促进骨关节炎状态下关节软骨修复再生的研究

项目编号： No.81470763

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 祝颂松

作者单位： 四川大学

项目金额： 73万元

中文摘要： 颞下颌关节（TMJ）骨关节炎（OA）是目前导致颞下颌关节功能障碍的主要疾病之一，而关节软骨的破坏是OA的最主要特征。如何阻止关节软骨破坏、恢复其正常形态结构与功能是目前制约治疗效果的主要瓶颈之一。研究表明在关节软骨组织中存在具有多能特性的干细胞，这一发现为OA病理机制的研究及其相关治疗提供了一个新的独特视角，但是目前的相关研究仍属空白。本项目研究内容主要包括下颌髁突软骨干细胞的分离、鉴定及其在OA状态下的性状改变；软骨干细胞与软骨细胞之间可能的内在关联机制；利用RNA干扰技术与基因芯片等技术筛选与调节可能参与调控软骨干细胞的关键靶蛋白与分子；利用软骨干细胞修复或逆转OA软骨病变等四方面。本项目的开展和实施不仅对深入阐明TMJOA疾病的细胞和分子机制具有重要的理论价值，而且可以为探索利用软骨干细胞，寻找提高OA治疗效果新的干预靶点提供重要线索。

中文关键词： 颞下颌关节；髁突软骨；软骨干细胞；软骨修复；骨关节炎

英文摘要： Temporomandibular joint (TMJ) osteoarthritis (OA) is one of the commonest diseases which result in TMJ dysfunctions. Damages of articular cartilage is one of the main characteristics in OA.Several methods have been developed for the treatment of TMJOA; however, the clinical outcomes remain dissatisfactory. Inhibition of cartilage damage and restoration of cartilage structure and fucntions may help improve the clinical outcomes. Recent studies showed that there are certain chondrocytes from human articular cartilage express markers specifi c for stem cells. These cells are called Chondrogenic Progenitor Cells (CPCs). The results may offer new insights into the pathogenesis and development of OA. However, no relevant study has been published yet. The mani goals of this project is to investigate identification, quantification and isolation of CPSs from articular cartilage in TMJ and it's characteristics in OA, the possible mechanism between CPCs and chondrocytes, isolation of the key protein and mollecullar regulating CPSs by RNA interference and gene microarray techniques and cartilage repair with CPCs. This opens new possibilities to achieve intrinsic cartilage repair and adds a novel aspect to the pathogenesis of cartilage degradation in arthritis. This project also lead to the development of new preventive and therapeutic agents to improve the quality of life for patients with osteoarthritis.

英文关键词： temporomandibular joint;condylar cartilage;chondrogenic progenitor cells;carilage repair;osteoarthritis