基于金三维纳米阵列电极的AFB1免疫传感体系构建和电化学机制研究

项目名称： 基于金三维纳米阵列电极的AFB1免疫传感体系构建和电化学机制研究

项目编号： No.21273056

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 曹立新

作者单位： 哈尔滨工业大学

项目金额： 77万元

中文摘要： 针对现有黄曲霉毒素B1（AFB1）分析方法灵敏度低、速度慢、操作步骤复杂等问题，提出结合金刷式三维纳米阵列电极（3DBNEEs）和无标记电化学免疫法的优势，构建AFB1检测体系。 在电极方面，结合模板法、化学沉积法和可控刻蚀技术，制备尺寸可控、性能稳定3DBNEEs。研究不同结构和尺寸的3DBNEEs体系中单个电极间扩散层的相互作用方式，及3DBNEEs体系的动力学特征。在传感器方面，重点研究抗体(抗原)在电极上的有效固定方法，探索抗体（抗原）固定的影响因素，揭示不同尺寸电极的生物分子识别膜对界面层电容、电化学探针的电极过程和动力学参数的影响规律。构建基于金3DBNEEs交流阻抗型AFB1无标记免疫传感检测技术体系，实现AFB1快速灵敏检测。本项的研究和实施不仅可为AFB1实际检测提供新的技术途径，也为3DBNEEs在免疫传感器中的应用提供科学依据，对保障国民食品安全与健康具有重要意义。

中文关键词： 模板法；三维纳米阵列电极；黄曲霉毒素1；无标记；免疫传感器

英文摘要： There are many shortcomings in the present analysis method, such as lack of sensitivity, time consuming, and too complicated to handle. The AFB1 detecting system have been suggested in this project by taking the combining advantages of gold 3D brush nanoelectrode ensembles （3DBNEEs）and label free electrochemical immunosensor. In the study of 3DBNEEs, the dimension controllable 3DBNEEs with high stability and reliability will be produced by effective combination of the technologies including template synthesis, electroless deposition and controllable etching. The interactional mode of the diffusion layer between the electrode element and the kinetic character were studied in 3DBNEEs system with different structures and dimensions. In the study of biosenor, the fixation method of the antibody (or antigen) on electrode and the influencing factor will be studied. The influence rule of the biological molecule recognition membrane on interfacial capacitance, electrode process and kinetic parameters of electrochemical probes will be revealed. The AC impedance label free immune biosensing technology system for the the detection of Aflatoxin B1 based on 3D gold electrode Ensembles will be constructed in order to achieve the purpose of AFB1 detection which possess the advantages of time saving and sensitivity. The study a

英文关键词： template synthesis；3D nanoelectrode ensembles；aflatoxin B1；label free；immuno sensor