以腹切米突变体为材料解析粳米腹白形成的生理与分子机制

项目名称： 以腹切米突变体为材料解析粳米腹白形成的生理与分子机制

项目编号： No.31470086

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 丁艳锋

作者单位： 南京农业大学

项目金额： 30万元

中文摘要： 垩白是一个由基因型与环境共同控制、涉及多种代谢与调节途径的复杂品质性状。以腹白为主的垩白米率较高已成为制约我国南方优质粳稻生产的关键障碍。本项目以申请者创制的高腹白腹切米突变体为材料，运用转录组学、蛋白质组学和代谢组学等系统生物学方法，通过突变体籽粒胚乳近胚端（垩白部位）和远胚端（透明部位）之间在碳、氮等代谢生理、活性氧清除、激素调节机制以及转录因子等方面的比较，在有效消除遗传背景和发育环境影响的基础上，精确解析调控腹白的基因网络、代谢途径和调控因子。研究结果可为垩白等品质性状的遗传改良和栽培调优提供有价值的参考，有助于破解垩白米率较高这一制约南方粳稻品质提升的重要难题。

中文关键词： 水稻；腹白；蛋白质组学；转录组学；代谢组学

英文摘要： Grain chalkiness is a complex trait as affected by interaction between genotype and environment and controlled by multiple regulatory pathways. High occurrence of white-belly grains has been one of the main obstacles for japonica rice production in southern China. This study aims to elucidate the physiological and molecular foundation of white-belly formation, using a notched-belly mutant with high rate of white-belly grains and employing the combination of system biology approaches including transcriptomics, proteomics, and metabolomics. In particular, effect of both genotype and growth environment can be minimized by comparison between the chalky part and translucent part of grains of the mutant. The resulting genetic networks and regulatory pathways or factors related to white-belly should be of significance for both rice improvement and cultivation practices aiming for high quality rice with lower chalky grain rate, and of practical value for japonica rice production in southern China.

英文关键词： Rice；White-belly；Proteomics；Transcripteomics；Metabolomics