化痰祛瘀息风法对幼龄癫痫大鼠血脑屏障通透性及相关蛋白表达影响的研究

项目名称： 化痰祛瘀息风法对幼龄癫痫大鼠血脑屏障通透性及相关蛋白表达影响的研究

项目编号： No.81460739

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 吴丽萍

作者单位： 甘肃中医药大学

项目金额： 45万元

中文摘要： 癫痫是一种严重危害人类健康的疾病，且儿童癫痫的发病率约为成人的10倍。中医药化痰祛瘀息风法治疗儿童癫痫具有有效、低毒副作用的优势，然而制约进一步稳定提高疗效的瓶颈在于化痰祛瘀息风法治疗癫痫的取效机制不明确，如化痰祛瘀息风法用药的活性成分能否通过血脑屏障作用于神经元，如何影响血脑屏障的功能等。本项目在前期临床和实验研究的基础上，通过观察幼龄癫痫大鼠血脑屏障超微结构、通透性的改变以及相关蛋白（MMP-9、ICAM-1、AQP-4、Cx43）的表达，探讨化痰祛瘀息风法对癫痫大鼠血脑屏障通透性及相关蛋白表达的影响，血脑屏障的破坏与癫痫发生发作的相关性，阐明化痰祛瘀息风法抗癫痫的作用机制和特点，以期指导临床，合理应用化痰祛瘀息风法防治小儿癫痫，稳定提高临床疗效。

中文关键词： 化痰祛瘀息风法；癫痫；血脑屏障

英文摘要： Epilepsy is a nervous system disease of serious harm to human health. The incidence of epilepsy in children is about 10 times that of adults. The method of resolving phlegm、removing stasis and calming wind is effective,the advantage of low toxicity and side effects in the treatment of epilepsy in children.But the mechanism of action is not clear about the method.For example, whether the active ingredient of the method through the blood-brain barrier(BBB) in neurons,how to affect the function of the BBB. On the basis of pre-clinical and experimental studies,through observing the BBB ultrastructure,permeability changes,and related proteins (MMP-9, ICAM-1, AQP-4, Cx43) expression in young epileptic rats, we will explore the impact of the method of resolving phlegm、removing stasis and calming wind on the BBB permeability and function in young epileptic rat, Investigating the relationship of the BBB and epilepsy, Clarifying the mechanism of action of this method antiepileptic.These study will help us to apply the method reasonablely,to improve the clinical efficacy in the prevention and treatment of pediatric epilepsy.

英文关键词： the Method of Resolving Phlegm;Removing Stasis and Calming Wind;Epilepsy;Permeability of Blood Brain Barrie