负载光敏药物生物自发光纳米胶囊的组装与光动力效应研究

项目名称： 负载光敏药物生物自发光纳米胶囊的组装与光动力效应研究

项目编号： No.21303219

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 赵洁

作者单位： 中国科学院化学研究所

项目金额： 25万元

中文摘要： 本项目申请主要是利用分子组装技术，制备生物自发光纳米胶囊并用其来携带光敏药物，研究该体系产生光动力效应的物理化学机理，为其在生物医药领域方面的应用提供重要的实验依据。主要研究思路是通过模板法和层层组装技术,将光敏药物与多孔模板共沉淀，并在其表面组装荧光素酶，除去模板后，获得载有光敏药物且能够被ATP诱导产生生物自发光的纳米胶囊。研究其生物自发光性、生物兼容性以及体系中单线态氧的量子产率，进一步通过体外细胞实验和移植性肿瘤动物实验研究载药纳米胶囊对细胞活性及繁殖能力的影响，确定其光动力效果。最终所获得的组装体期望能够直接应用于临床的光动力治疗，为开发具有功能性的高效率光动力治疗药物制剂提供创新思路。

中文关键词： 功能化纳米结构；分子组装；光动力疗法；联合治疗；单线态氧

英文摘要： In this project, bioluminescent nanocapsules were fabricated via molecular assembly for the delivery and activation of photosensitizers. The photodynamic effect and relevant physichemical mechanisms of these nanocapsules were studied, to provide a practical basis for the application in the field of biomedicine. In detail, photosensitizers were coprecipitated with mesoporous templates, and luciferase was assembled onto their surface via a layer-by-layer technique. After the removal of templates, nanocapsules uploading photosensitizers which could emit visible light in the presence of ATP were obtained. Experiments will be carried out to investigate the bioluminescent property, biocompatibility, and singlet-oxygen quantum yield. Then, experiments in vitro and in vivo were employed to demonstrate the effect of photosensitizer-loaded nanocapsules on the activity of tumor cells and cell reproduction capacity. The nanocapsules are expected to be applied in clinical photodynamic therapy of cancers, providing an innovative approach for assemblying functional new drug carriers with high efficiency.

英文关键词： Functional nanostructures；Molecular assembly；Photodynamic therapy；Combined therapy；Singlet oxygne