番茄广谱胁迫蛋白SlUSP介导抗灰霉病的分子机制

项目名称： 番茄广谱胁迫蛋白SlUSP介导抗灰霉病的分子机制

项目编号： No.31272181

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 王涛涛

作者单位： 华中农业大学

项目金额： 80万元

中文摘要： 前期我们在番茄中克隆了一个SlUSP基因，在番茄中超量表达后其非生物抗性显著提高，该基因与GenBank数据库中的基因同源性最高仅为58%，目前在植物中未见有功能的USP基因的报道。研究期间发现超量表达SlUSP的植株对灰霉病抗性明显强于对照，表明其能同时介导非生物与生物抗性信号。因此，本研究将从能同时介导非生物与生物抗性的信号因子Ca2+，ROS及激素SA，JA和ET等入手，利用这些信号因子及其抑制剂进行处理，研究由SlUSP介导抗病性提高的主要信号传导途径。运用高通量RNA-Seq技术，在接种灰霉病前后，从全基因组水平对转基因株系和对照的表达谱进行分析，探寻SlUSP介导的信号影响到哪些信号因子或激素的合成，最终激活了哪些抗病相关的基因。进一步，利用酵母单杂交技术，挖掘调控该SlUSP基因的转录因子或调节基因。该研究有助于揭示植物抗病的新机制，理解生物逆境与非生物逆境的网络关系。

中文关键词： 番茄；SlUSP；灰霉病；激素；分子机理

英文摘要： SlUSP was cloned from tomato in our previous research, the abiotic tolerance was significantly enhanced in overexpressing SlUSP of tomato, the gene homology is just 58%, when blasted against the GenBank database, and no functional USP genes was reported in plant in the present. The disease resistance was also enhanced significantly in overexpressing SlUSP plants compared to the control when exposed Botrytis cinerea, indicating that SlUSP probably mediated the abiotic and biotic resistance. So we start the study from the signal factors related to the abiotic and biotic resistance, such as Ca2+, ROS and some hormones SA, JA and ET and so on. In order to determine the main signal factors mediated by SlUSP, the transgenic and wild type plants would be treated with hormones or hormone inhibitors, and the expression profile of transgenic and wild type plants would be investigated from the whole genomic level after inoculating Botrytis cinema using RNA-Seq technology, there studies will help to explore which signal and hormone biosynthesis pathway were mediated by SlUSP, and which disease resistance genes were activated finally. Moreover, Yeast one hybrid technology was performed to explore the transcription factors and regulated genes interacted with the promoter elements of SlUSP. This study helps to reveal the new

英文关键词： Tomato；SlUSP；Gray mold；Hormone；Molecular mechanism