广西北部湾红树林根际放线菌生物多样性及其次生代谢产物的研究

项目名称： 广西北部湾红树林根际放线菌生物多样性及其次生代谢产物的研究

项目编号： No.31260004

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 姜明国

作者单位： 广西民族大学

项目金额： 49万元

中文摘要： 海洋放线菌的多样性与其次生代谢产物的化学多样性是密切相关的，所以海洋放线菌已经成为人类寻找具有生物活性新化合物不可替代的资源。地处亚热带的广西北部湾海域是迄今保护最好的红树林生态系统之一，孕含丰富的根际放线菌资源。本申请拟采用原位培养、实验室培养和免培养的方法，对广西北部湾海域红树林根际放线菌的生物多样性进行研究。优化原位培养与实验室培养方法，旨在尽可能多的分离放线菌菌株包括稀有放线菌，初步建立菌种资源库。进一步通过波谱手段与BOX-PCR方法排除重复菌株后，利用16SrDNA序列进行分子聚类和系统发育分析，全面了解该地区放线菌资源的多样性。对于新种进行多相分类鉴定。在生物活性的指导下，利用理化性质及波谱学方法对分离的单体化合物进行结构解析，初步研究放线菌次生代谢产物的化学多样性。对及时开发和保护广西北部湾海域海洋放线菌资源具有重要意义。

中文关键词： 海洋放线菌；红树林；根际微生物；原位分离方法；多样性

英文摘要： Mangrove forests are unparalleled productive ecosystems of intertidal coasts in tropical and subtropical coastal districts. The ecosystems exhibit characteristics "high salinity, extreme tides, strong winds, high temperature and muddy, anaerobic soils". Nowdays, more and more reports imply that actinomycetes population play important ecological roles considered as one of the major group of the unique habitat. The mangrove environments has become an invaluable source for discovering novel actinomycetes hence new chemical diversity. So, a well-defined biodiversity and taxonomic study of actinomycetes is valuable to understand actinomycetes from the mangroves environment.Actinobacteria play an important role in life cycles in the cycling of organic matter in the soil ecosystem. Actinomycetes maintain a distinguished role due to their diversity and proven ability to produce novel compounds, so the discovery of new actinomycetes, especially of rare actinomycetes, is becoming increasingly important. Many studies have been done to isolate and identify novel actinomycetes by conventional Petri dish plating. Indeed, the tranditional methods are steadily out of action, which is likely due to repeated isolation and screening of the identical actinomycetes, the rediscovery of ordinary antibiotics from cultivable species is

英文关键词： marine actinomycete；mangrove；rhizosphere microorganism；in situ cultivation；biodiversity