大豆耐盐相关基因GmNACx在大豆耐盐性中的作用机制研究

项目名称： 大豆耐盐相关基因GmNACx在大豆耐盐性中的作用机制研究

项目编号： No.31201269

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 李朔

作者单位： 山东大学

项目金额： 23万元

中文摘要： 大豆（Glycine max L.）是重要的粮食及经济作物，耐盐性较差。大豆基因转化较难，有关耐盐基因在大豆耐盐中的作用研究未见报道。本实验室建立了一种高效的大豆转化技术，筛选出耐盐大豆- - 圣豆9号，建立了其在盐及非盐胁迫下的表达谱，比较了它们的表达差异，筛选出NAC转录因子基因GmNACx，并获得其过表达转基因大豆。在此基础上，本项目拟开展GmNACx的耐盐作用机制研究。包括亚细胞定位、转录激活活性、组织特异性表达及其在盐和激素处理下的表达变化；GmNACx RNAi转基因大豆的获得及分子鉴定，两种转基因大豆耐盐性、生理指标及耐盐相关基因表达分析，确定该基因耐盐功能；分析转基因大豆及对照转录组差异，结合ChIP-sequence，筛选其下游靶基因，进行EMSA、ChIP等验证，以期揭示该基因调控大豆耐盐的分子机制，为创建转基因耐盐大豆新种质奠定基础。

中文关键词： 大豆；转基因；NAC；转录因子；

英文摘要： Soybean (Giycine max L.) is one of the most important food and economic crops and has not high salinity tolerance. So far, it is hard to perform the soybean transgene and was not reported about the funtional analysis of salt tolerance related soybean genes in soybean itself. An efficient soybean transgenic technique has been established in our lab and a sanility tolerant soybean cultivar SHENGDOU No.9 was selected from 179 cultivars. A transcriptomic comparison was performed in SHENGDOU No.9 between salinity-stressed and non-stressed treatments and several salt responsive genes were selected as candidate salinity tolerance related genes.GmNACx,one of the NAC transcription factors,was further cloned and over expressed in soybean and the T3 transgenic seed have been obtained. Based on these working foundations, this project is to study the function and molecular mechanism of GmNACx in salinity tolerance in soybean. The contents of the project include: protein sub-cellular localization, trancativation, gene expression patterns in different tissues and in response to abiotic stresses and plant hormores; the obtainment of GmNACx over-expression and RNAi transgenic soybean seedlings and their validation with Southern-blot and qRT-PCR, the salinity tolerance and physiological index of the transgenic seedlings, to make

英文关键词： soybean；transgene；NAC；transcription factor；