光催化活性氧胁迫下的细胞应激反应机制研究

项目名称： 光催化活性氧胁迫下的细胞应激反应机制研究

项目编号： No.U1504311

项目类型： 联合基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 张静涛

作者单位： 郑州轻工业学院

项目金额： 27万元

中文摘要： 二氧化钛（TiO2）在光的激发下生成的载流子能够与H2O、O2等反应产生对细胞具有胁迫作用的活性氧（ROS）并导致其死亡。细胞作为生命有机体能够对此胁迫做出相应的应激反应，我们前期的研究发现光催化能够导致酿酒酵母菌液泡发生应激变化，然而细胞在此胁迫下的应激反应机制尚未明确。本项目以大肠杆菌和酿酒酵母菌为研究对象，拟通过筛选改性TiO2光催化反复诱导的大肠杆菌和酿酒酵母菌，以期望获得光催化活性氧诱导的抗氧化突变体。应用数字化基因表达技术（DGE）和同位素相对标记与绝对定量技术（iTRAQ）对筛选到的突变体和光催化处理后半数致死状态下的细胞进行转录组（大肠杆菌和酿酒酵母菌）和蛋白组（酿酒酵母菌）差异分析，揭示光催化活性氧胁迫下细胞发生的主要应激反应以及不同细胞主要应激反应的异同。本研究预期将有助于明确半导体光催化导致细胞死亡的机制，为光催化杀菌提供理论基础，也为光催化杀菌材料合成提供指导。

中文关键词： 光催化；活性氧；数字化基因表达；同位素相对标记与绝对定量；细胞应激反应

英文摘要： Charge carriers could be generated by the light excitation of the titanium dioxide (TiO2). And the cell could be killed due to the oxidative stress of the reactive oxygen species (ROS), produced by the charge carriers with the reaction of water, oxygen and so on. However, as living organism, cells could have the corresponding stress response under this situation. In our previous work, stress reaction was observed in the vacuoles of Saccharomyces cerevisiae induced by the photocatalysis. However, the mechanism of cellular stress response is unclear. Here we use Escherichia coli and S. cerevisiae as model systems. We expect to obtain antioxidant mutants by screen out the E. coli and S. cerevisiae, induced by ROS photocatalysed of modified TiO2 repeatedly. Digital Gene Expression (DGE) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) were used to analyze the transcriptome (E. coli and S. cerevisiae) and proteome (S. cerevisiae) of both the mutants and the cell in semi-lethal condition by the photocatalysis treatment, respectively. Through analyzing the difference of gene transcription and expression profiles, the main stress response of cellular treated by the photocatalysis will be indicated, similarities and differences between different cells will also be elucidated. The research will help to understand the disinfection mechanism of the photocatalysis, and in theory set an example for the photocatalytic sterilization, as well as offer guidance for the synthesis of the photocatalytic bactericidal materials.

英文关键词： photocatalysis;reactive oxygen species ;digital gene expression;isobaric tags for relative and absolute quantitation;cellular stress response