新疆特色植物药桑降血糖活性成分及协同效应研究

项目名称： 新疆特色植物药桑降血糖活性成分及协同效应研究

项目编号： No.31460080

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 杨玲

作者单位： 塔里木大学

项目金额： 50万元

中文摘要： 药桑(Morus nigra L.)是新疆在全国独一无二的、在自然界极为罕见的珍稀药用黑果桑资源。现代药理研究表明，药桑桑叶含有丰富的黄酮、生物碱、多糖等生物活性物质，对糖尿病具有一定的预防和治疗作用。然而，其活性成分及相互协同增效关系尚不清楚。 本研究以药桑桑叶为材料，通过对黄酮、生物碱和多糖的提取及系统分离、精制，以三种活性成分的总含量结合α-葡萄糖苷酶抑制剂体外筛选模型活性跟踪，建立药桑桑叶降血糖主要活性成分集成提取工艺；通过高速逆流色谱（HSCCC）、制备液相色谱（PHPLC)等色谱分离技术获得单体化合物，对获得的单体或单体组合物进行糖尿病小鼠体内活性筛选；在体内外活性筛选实验评估，获得具有降血糖功能的活性单体或组合物的基础上，依据中效原理，采用CompuSyn软件，进一步探讨其降血糖活性的协同增效关系。本研究为药桑资源的高效利用和相关功能性生物制品的研制开发提供实验支持。

中文关键词： 药桑；降血糖；活性成分；协同作用

英文摘要： Yao sang (Morus nigra L.) in Xin Jiang, which is a unique in country, belong to a very rare medicinal mulberry resources (Morus nigra L.) . Modern pharmacological studies have shown its leaves are rich in flavonoids, alkaloids, polysaccharides, and other biologically active substances, which have play a role of prevention and treatment of diabetes. However, it is still unclear about its active ingredient and synergistic effect. The mulberry leaf from yaosang (Morus nigra L .) was selected as materials in this project, its main hypoglycemic active ingredients include of flavonoids, alkaloids and polysaccharides were extracted, separated and purified. Bases on the total content of the three kinds of active ingredients and the results of activity tracking of a-glucosidase inhibitor in vitro screening model, The integrated extraction processing of hypoglycemic active ingredients from mulberry leaf were established. The monomer compound could be obtained by the mean of the high-speed countercurrent chromatography(HSCCC),preparation performance liquid chromatography (PHPLC) and other chromatographic separation technology. According to the activity experimental evaluation of diabetic mice screening model in vivo and a-glucosidase inhibitor in vitro screening model, the active ingredient with a hypoglycemic function could be obtained, based on Chou-Talalay method, using CompuSyn soft, Exploring the synergistic effect of its hypoglycemic activity. The results of this study could provide experimental support for yaosang in the way of efficient use and the related functions products development.

英文关键词： yaosang(Morus nigra L.);hypoglycemic;bioactive component;synergistic effect