新型非病毒基因载体PEI-壳聚糖-靶向肽对肿瘤干细胞上的靶向研究及其免疫基因治疗

项目名称： 新型非病毒基因载体PEI-壳聚糖-靶向肽对肿瘤干细胞上的靶向研究及其免疫基因治疗

项目编号： No.81201771

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学1

项目作者： 赵青青

作者单位： 重庆医科大学

项目金额： 22.5万元

中文摘要： PEI和壳聚糖是优秀的非病毒基因传递系统，但都存在其不足性。为了改善壳聚糖较低的转染效率，我们将其与低分子的PEI通过化合作用连接在一起，以期望得到高效安全的非病毒基因载体材料。材料的组成，粒径，电位等物理化学性质将被研究，同时转染效率以及细胞毒性也会在实验中被评估。近期，肿瘤干细胞是研究的热点，在本次课题中，会筛选适宜的肿瘤干细胞的靶向物，如小分子的多肽。将多肽与先前合成的新型材料再结合一起，就可能得到靶向肿瘤干细胞的非病毒载体。我们将会评价该载体针对肿瘤干细胞的特异性，分为体外及体内两块来研究。最终将携带治疗基因，希望能实现对肿瘤干细胞的治疗效果。

中文关键词： 肿瘤干细胞；CD133；靶向；非病毒载体；

英文摘要： PEI and chitosan are considered to be promising non-viral gene delivery vectors. To improve the transfection efficiency of chitosan, we would link chitosan with polyethylenimine (PEI,Mw= 1.8 kDa) by 1,1_-carbonyldiimidazole to form a complex. The composition, particle size, as well as the zeta potential of this chitosan-linked-PEI (CP) complex will be measured. And the DNA binding ability, cytotoxicity, and gene transfection efficiency of CP complex will also investigated in cancer cells.Recent studies elucidated the presence of cancer stem cells that have the exclusive ability to regenerate tumors. These cancer stem cells share many characteristics with normal stem cells, including self-renewal and differentiation. With the growing evidence that cancer stem cells exist in a wide array of tumors.The concept of cancer stem cells may have profound implications for our understanding of tumor biology and for the design of novel treatments targeted toward these cells.In this research we also want to link CP and some peptide which target to tumor stem cells,and investigate their physico-chemical properties as well as the biochemical propertyies.The threapy effect which induced by the target materials is our final hope.

英文关键词： tumor stem cells；CD133；target；non-viral vector；