黄酮类物质抑制膳食中杂环胺产生的作用机理研究

项目名称： 黄酮类物质抑制膳食中杂环胺产生的作用机理研究

项目编号： No.31471669

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 张燕

作者单位： 南开大学

项目金额： 80万元

中文摘要： 杂环胺是富含蛋白质的食品在热加工过程中产生的一类准致突变和致癌化合物，广泛存在于日常饮食中，是一种潜在的食品安全危害因子，因此膳食中杂环胺控制技术的相关研究具有重要意义。课题将在前期研究证明黄酮类物质对食品体系中杂环胺具有较强抑制作用的基础上，通过化学模拟体系，研究黄酮添加量与杂环胺抑制率之间的量效关系，分析建立黄酮结构参数与杂环胺抑制率之间的构效关系，探讨不同结构黄酮及添加量下杂环胺抑制率与黄酮自由基清除力之间的关系，并采用量子化学计算与反应产物结构鉴定相结合，研究杂环胺的产生途径及黄酮抑制杂环胺生成的反应途径，最终揭示热加工过程中黄酮类物质抑制杂环胺产生的作用机制，为实现食品加工过程中杂环胺的有效控制提供理论和应用基础。

中文关键词： 杂环胺；黄酮；抑制作用；形成机理

英文摘要： Heterocyclic amines are pre-mutagenic and carcinogenic compounds produced during the thermal processing of food which is rich in proteins. Heterocyclic amines are widespread in diets, becoming a potential risk factor in food safety, so it is important to investigate the formation mechanism of heterocyclic amines and their control techniques in foods. Based on the pre-investigated results that flavonoids had an obvious inhibitory effect on the formation of heterocyclic amines, the study will investigate inhibitory mechanism of flavonoids under simulation model which produces the heterocyclic amine. Through adding flavonoids in the model system, the dose-effect relationship between the dosage of flavonoids and the inhibition ratio on heterocyclic amine, the structure-activity relationship between the configuration of flavonoids and its inhibition ratio on heterocyclic amines, the relationship between the free radical-scavenging ability of flavonoids and its inhibition ratio on heterocyclic amines will be established. By quantum chemical calculation and structure analysis of intermediates produced in the reaction system, the formation pathway of heterocyclic amine and the inhibitory pathway of flavonoids on heterocyclic amines in thermal processing will be revealed. The inhibitory mechanisms of flavanoids on the formation of heterocyclic amines will provides theoretical foundation of applicable techniques for controlling heterocyclic amines in food processing.

英文关键词： heterocyclic amine;flavonoid;inhibitory effect;formation mechanism