新型稀土配合物和上转换纳米晶掺杂的Ag@SiO2荧光纳米粒子的制备及其生物医学应用

项目名称： 新型稀土配合物和上转换纳米晶掺杂的Ag@SiO2荧光纳米粒子的制备及其生物医学应用

项目编号： No.21271126

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 尹东光

作者单位： 上海大学

项目金额： 80万元

中文摘要： 稀土配合物和上转换纳米晶具有较大的Stokes位移和长荧光寿命等独特优点，但其荧光强度不够高，限制其应用与发展，而构建它们掺杂的核壳型Ag@SiO2纳米粒子，利用纳米银的金属增强荧光效应及硅壳的作用，可以显著提高它们的荧光强度和光稳定性。本研究在合成新的稀土配合物及上转换纳米晶的基础上，将石墨烯引入金属纳米结构，构建崭新的金属增强荧光纳米结构，通过两种新制备方法，分别制备新型稀土配合物及上转换纳米晶掺杂的Ag@SiO2纳米粒子，研究其组成、结构、性能及相互间的关系与规律，探究新方法的荧光增强机制,最后将制备的纳米粒子作为新型荧光探针应用于生物医学研究。通过该课题的研究，制备出新型、荧光强度高、光稳定性好、可以直接应用于生物医学研究的稀土荧光纳米粒子，解决目前稀土荧光探针存在的不足，探索制备新型稀土荧光探针的新方法。该项目的实施既有重要的科学意义，又有广阔的应用前景。

中文关键词： 稀土配合物；稀土上转换纳米晶；提高荧光强度；金属增强荧光；石墨烯

英文摘要： Rare earth complexes and upconversion nanocrystals have unique advantages，such as large Stokes shift and long lifetime. However, their weak fluorescent intensities limit their applications. The core/shell Ag@SiO2 nanoparticles doping with rare earth complexes or upconversion nanocrystals can significantly improve their fluorescent intensities due to the functions of metal enhanced fluorescence and the silica shell. In this study, we will prepare new rare earth complexes and upconversion nanocrystals, then fabricate a novel nano-structure of metal enhanced fluorescence by introducing graphene, and synthesize novel rare earth complexes and upconversion nanocrystals-doped core/shell Ag@SiO2 nanoparticles through new methods, respectively. Subsequently, the structures and properties of the nanoparticles, the fluorescence enhanced mechanisms of the new methods, and the applications in biological medicine of the nanoparticles, will be investigated. Through this study, the desired rare earth fluorescent nanoparticles which are novel, strong fluorescent, high photostable and applicable in biological medicine, will be obtained. Moreover, it is promise to develop new methods to prepare novel fluorescent probes which are advantageous over the existed probes. This work can contribute greatly to research in nanoscience and h

英文关键词： rare earth complexes；rare earth upconversion nanocrystals；improving fluorescent intensity；metal-enhanced fluorescence；graphene