近红外菁染料聚集调控及其用于生物分子检测研究

项目名称： 近红外菁染料聚集调控及其用于生物分子检测研究

项目编号： No.21476185

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 有机化学

项目作者： 徐勇前

作者单位： 西北农林科技大学

项目金额： 86万元

中文摘要： 很多生物分子与疾病的发生存在着密切的关联，开发能检测这些生物分子和其浓度变化的荧光传感器，为相关疾病的诊断提供预警，具有极其重要的意义。方酸菁染料易发生多种聚集，不同的聚集具有不同的光谱特征。本项目拟设计合成系列结构不同的方酸菁染料化合物，构建染料聚集状态可调控的分子自组装体，研究其构效关系和生物分子对其聚集状态的调控，双激发比例荧光用于生物相关疾病诊断。此外受纳米胶束可作为药物分子传输载体的启示，在检测溶液中引入两亲分子和高分子聚合物来调控染料的聚集状态，希望通过引入疏水作用、π-π相互作用、静电效应等协同作用来增大生物分子对染料聚集的调控能力，达到选择性识别生物分子的目的。通过这项研究，寻找到一种普适的用于生物分子检测的方法，为疾病相关的生物分子诱导药物分子释放提供理论指导，推进易聚集的近红外荧光染料在生物医药领域的应用。

中文关键词： 近红外菁染料；聚集体；生物分子检测；比例荧光；胶束

英文摘要： Many biomolecules are closely associated with the diseases, thus it is of very importance to explore fluorescent sensors capable of detecting these biomolecules, determining their concentrations and providing early clue of the diagnosis of related diseases. Squaraine are a class of dyes that easily form aggregates with different spectral features in aqueous solution. This project aims to design and synthesis of a series of squaraine dyes to construct self-assembly of dye aggregates. In virtue of this system, the relationship between structure and efficiency, and the regulation by biomolecules with ratiometric fluorescence for the diagnosis of biological related disease will be comprehensively investigated. In addition, inspired by the concept that nano-scaled micelles can be used as drug carriers, amphiphilic molecules and polymers are introduced to this array to adjust the aggregations of dyes. Through the introduction of synergistic effect including hydrophobic, π-π and electrostatic interactions to increase the regulation capability of biomolecules toward dye aggregates, the selective recognition of biomolecules is realized. Through this study, a universal strategy for the detection of biomolecules will be set up. It will provide theoretical guidance for drug release triggered by biomolecules that associated with diseases, and promote the application of other near infrared fluorescent dyes in biomedicine area.

英文关键词： Near infrared fluorescent squaraine dyes;Aggregates;Biomolecules detection;Ratiometric fluorescence;Micelles