基于代谢组学—蛋白质组学联用的冠心病血瘀证个体化研究

项目名称： 基于代谢组学—蛋白质组学联用的冠心病血瘀证个体化研究

项目编号： No.81202647

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 简维雄

作者单位： 湖南中医药大学

项目金额： 23万元

中文摘要： 辨证不仅要确定证的有/无，还要辨不同患者证的轻重程度- - 个体化，因以往对证个体化的思辨过程多具有主观性。为此，本项目依据血瘀证症征计分标准采用聚类分析，建立冠心病血瘀证轻、中、重三个亚型；在已建立的代谢组学技术平台上确立血瘀证亚型代谢表型，再运用蛋白质组学筛选血瘀证亚型差异功能蛋白，通过化合物-蛋白质（酶）-化合物的链级反应，应用数据挖掘技术进行亚型-代谢产物-蛋白质的关联性分析，运用生物信息学方法探索冠心病血瘀证个体化病理机制本质为辨证论治、随症加减的治疗精髓提供理论依据，为基于现代技术的中医个体化医学奠定基础。

中文关键词： 血瘀证；个体化；蛋白质组学；代谢组学；

英文摘要： Dialectical not only to determine the license with or without，but also to distinguish the different severity of patient cards - individual, Due to the past, check against individual speculative process is subjective.To this end, the project is using cluster analysis based on blood stasis disease symptoms scores can range Establishment of Blood Stasis Syndrome in Coronary heart Disease light, moderate and severe three subtypes;Established metabolomics technology platform to establish the subtype metabolic phenotype of blood stasis,Proteomic screening blood stasis subtype differences in functional protein,Through the chain-level response of the compound - protein (enzyme) - compounds,Data mining techniques subtypes - metabolites - analysis of proteins associated Bioinformatics methods to explore the nature of the pathological mechanism of Blood Stasis Syndrome in Coronary heart Disease individualized for diagnosis and treatment, to provide a theoretical basis for the treatment of the essence of addition and subtraction with the disease,To lay the foundation for modern technology-based Chinese medicine individualized medicine.

英文关键词： Blood stasis syndrome；Individual；Proteomics；Metabolomics；