超顺磁性SERS活性基底材料的可控合成及其在肿瘤细胞检测中的应用

项目名称： 超顺磁性SERS活性基底材料的可控合成及其在肿瘤细胞检测中的应用

项目编号： No.21301179

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 高倩

作者单位： 中国科学院合肥物质科学研究院

项目金额： 25万元

中文摘要： 由于具有高灵敏度和光谱选择性，SERS免疫检测在肿瘤诊断方面具有潜在的优势。而SERS活性基底的构筑是实现拉曼增强的前提，多功能性是提高SERS检测可靠性的有效途径。本项目拟结合铁基纳米粒子的分离富集特性和贵金属纳米粒子的优异光学性质，设计和制备磁性-贵金属复合功能纳米材料。利用铁氧化物的特殊形状作为模型，从理论上分析可能的SERS增强机理，研究材料的磁性对SERS增强效应的影响，为可控合成高效的磁性SERS活性基底材料提供实验和理论依据。得益于此类材料的高SERS效应的调谐，通过解决因表面包覆、修饰和联接功能分子后所导致复合材料的磁响应性能以及SERS性能减弱等问题，构建基于SERS的集靶向、分离、检测等多功能于一体的生物探针，将其用于肝癌标志物甲胎蛋白的快速分离与超灵敏检测，为实现全血中肿瘤的早期诊断提供科学依据和理论基础。

中文关键词： 磁性；复合材料；可控构筑；检测；催化

英文摘要： With high sensitivity and spectral selectivity, surface-enhanced Raman scattering (SERS) labeled immunoassay has potential advantages in tumor diagnosis. But the construction of active SERS substrate is essential for obtaining the enhanced Raman signals and multifunction is the effective way to improve the reliability of SERS detection. This project aims to design and prepare magnetic-noble metal nanocomposite materials, which combine the separation and enrichment characteristics of iron series nanomaterials with the excellent optical properties of noble metal nanoparticles. In order to investigate the role of magnetic materials in the SERS enhancement effect of noble metal nano-layers, special shape of iron oxides are used and the enhancement mechanism are also discussed. These results provide experimental and theoretical basis for the controlled synthesis of highly efficient magnetic and SERS active substrate materials. Thanks to the tuning of such composite materials, SERS-based biological probes with target, separation, detection and other functions are constructed. Thus, the following two problems can be solved, namely, delayed magnetic response of composite materials as well as weakened SERS performance, which are caused by the surface coating, modification and coupling of functional molecules. Such probes

英文关键词： Magnetic；Composite material；Controllable construction；Detection；Catalysis