Ti-Ag 纳米管/HA 复合涂层的抗菌性能及生物相容性的基础研究

项目名称： Ti-Ag 纳米管/HA 复合涂层的抗菌性能及生物相容性的基础研究

项目编号： No.81501857

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 张杭州

作者单位： 中国医科大学

项目金额： 18万元

中文摘要： 目前临床上使用的金属内植物(钛金属及其合金)缺乏长期抗菌性能及优越的体内生物相容性（骨整合），本研究组以阳极氧化技术制备钛银合金表面纳米管。通过在模拟体液中在纳米管表面沉积钙磷涂层（即羟基磷灰石）。联合应用钛银纳米管/HA复合涂层优势 :1.低剂量银离子能达到长期抑制假体周围细菌粘附增殖(缓释银离子)；2.表面纳米管能够促进骨组织生长；3.纳米管具有抑制细菌粘附的作用。本研究将利用扫描电镜对其进行表征分析检测；在动物模型中进行银离子释放研究；评价钛银合金纳米管组织相容性、细胞毒性及抗菌性能。检测大兔模型检测假体周围骨长入，成骨相关基因及内植物与周围骨的结合力等。骨长入情况及内植物与周围股情况等通过BIC试验及拉力试验（pull out test) 检测，同时内植物周围组织的成骨相关基因（OPN,OCN, COL-1, ALP)等通过实时定量PCR技术检测。我们将探索其抗菌性能及应用基础研究

中文关键词： 纳米结构；纳米羟基磷灰石；银；生物相容性；抗感染

英文摘要： Although Titanium (Ti) and its alloys are widely used clinically, they have some defects such as long-term antibacterial properties (inhibit biofilm formation and prevent implant associated infection) and well biocompatibility in vivo (osseointegrity). The nanotubes assay was fabricated on the surface of the Ti-Ag alloy by anodizing technology and the HA was coated on the surface of Ti/Ag nanotube in simulated body fluid (SBF). Ti-Ag nanotube/HA surface will give some advantages; such as 1) The reliable release and long-term antibacterial ability due to sustained release of Ag ions. 2) The nanotubes which were fabricated on Ti-Ag alloy can promote bone activity. 3) The nanotubular surface can reduce bacteria adhesion. In this study, the surface of nano-structured Ti-Ag/ HA is studied by scanning electron microscopy, the Ag ion release kinetics was studied in animal models to evaluate the Ag ion release mechanism in vivo; the biocompatibility, cytotoxicity and antibacterial properties of nano-structured surface of Ti-Ag nanotube/HA also studied in vivo and in vitro. The biocompatibility of Ti-Ag nanotubes/HA in rabbit model by studying the gene expression, bone formation and shear strength around the implants. The Ti-Ag nanotube/HA, Ti-nanotube and Cp-Ti were implanted into femur of the rabbits. Mechanical fixation of the peri-implant-bone area and bone contact were evaluated by pull-out tests and histology, and the gene expression of alkaline phosphatase(ALP), OPN, OCN and collagen-I (Col-I) was examined by using real-time PCR after implantation. The study will develop new nanostructured Ti-Ag nanotube/HA and explore the application for biomaterial for preventing postoperative orthopedic implant-related infection

英文关键词： nano-structure;nano-Hydroxyapatite;silver;biocompatibility;antibacterial ability