海岛棉GbHyPRP1基因抗黄萎病功能鉴定及抗病分子机制初探

项目名称： 海岛棉GbHyPRP1基因抗黄萎病功能鉴定及抗病分子机制初探

项目编号： No.31301370

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 杨君

作者单位： 河北农业大学

项目金额： 23万元

中文摘要： 棉花黄萎病是一种重要的土传真菌维管束病害，严重影响棉花产量和纤维品质。抗黄萎病遗传改良和抗病机制研究一直是棉花遗传育种领域的重大科学问题。项目组首次克隆获得海岛棉GbHyPRP1基因，其参与棉花抗黄萎病过程，是一种潜在的抗病因子。为探究GbHyPRP1抗黄萎病功能与分子机制，本项目拟进行以下研究：一，从不同抗、感棉花品种克隆HyPRP1基因及启动子，明确基因序列差异与品种抗病性的关系；二，采用荧光实时定量PCR和免疫电镜技术，分析黄萎病菌胁迫下基因和蛋白在不同抗、感棉花品种中的表达差异；三，遗传转化陆地棉品种和拟南芥，明确GbHyPRP1基因抗黄萎病功能；四，利用串联亲和纯化技术从棉花蛋白组中捕获GbHyPRP1互作蛋白，筛选介导GbHyPRP1抗病反应的重要因子，分析蛋白互作的抗病意义。项目结果将不仅为棉花抗黄萎病机制研究提供理论依据，还将对棉花抗病分子育种具有重要的理论和应用意义。

中文关键词： 棉花；黄萎病；GbHyPRP1；功能鉴定；分子机制

英文摘要： Verticillium wilt in cotton caused by Verticillium dahliae is a soil-borne vascular disease, which results in serious loss in yield and fiber quality. Both genetic improvement and research on the resistance mechanism of cotton to V. dahliae are always important scientific problems in breeding and genetics. We have firstly cloned a GbHyPRP1 gene from sea-island cotton, Gossypium barbadense. GbHyPRP1, which take part in resistance of cotton to V. dahliae, is a potentially resistance factor. In order to character and study on the molecular mechanism of GbHyPRP1 involved in resistance of cotton to verticillium wilt, in this project the research is as following: First, we will definitely show the relationship between resistance and HyPRP1 sequence, which contain genes and promoters from different resistant and susceptible cotton varieties; Secondly, we will analyze the expression of HyPRP1 gene and protein in different resistant and susceptible cotton varieties induced with V. dahliae by real-time quantitative PCR and immune electron microscopy; Thirdly, the resistance of GbHyPRP1 to verticillium wilt was character by transformation of upland cotton (Gossypium hirsutum L.) and Arabidopsis thaliana; Fourthly, we will capture GbHyPRP1-interacting proteins in using tandem affinity purification and screen important fact

英文关键词： cotton；verticillium wilt；GbHyPRP1；functional characterization；molecular mechanism