基于滑动窗口组件式长链RNA结构预测模型的lncRNA胃癌耐药研究

项目名称： 基于滑动窗口组件式长链RNA结构预测模型的lncRNA胃癌耐药研究

项目编号： No.61471181

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 无线电电子学、电信技术

项目作者： 朱晓冬

作者单位： 吉林大学

项目金额： 80万元

中文摘要： 影响胃癌治疗疗效的关键因素在于多药耐药。胃癌耐药发生机制复杂，涉及的分子具有多样性，针对任何单一耐药蛋白的干预都难以有效逆转胃癌耐药。lncRNA对耐药蛋白调控具有高特异性，是新的可克服胃癌耐药难题的切入点。但胃癌耐药中特异表达的lncRNA数量众多，传统生物实验逐条敲除、抑制的研究方法消耗大量时间、物力，实际操作性差。而生物信息学科与生物学科的深度合作研究解决这一难题面临的障碍是：lncRNA的结构特征与其生物功能密切相关，获得lncRNA生物功能的关键在于其二级结构，目前二级结构预测软件局限于关注全局结构的准确性，忽略了更为重要的局部结构保守的功能组件，也无法满足lncRNA结构功能的长度需求。因此，本项目提出一种基于滑动窗口的组件式lncRNA结构预测模型来指导胃癌耐药生物实验，为利用lncRNA逆转、终止胃癌耐药提供可操作的有效途径，也为克服其他癌症耐药难题研究提供借鉴。

中文关键词： 生物信息处理；胃癌；多药耐药；lncRNA；RNA二级结构

英文摘要： The key factor affecting the therapeutic efficacy of gastric-cancer is multi-drug resistance.Because the drug-resistance mechanism of the gastric-cancer is complex and the involved molecular have diversity, intervention targeting at anyone resistance protein is hard to reverse the drug-resistance of gastric-cancer.LncRNA can regulate drug-resistance protein specifically and is a new breakthrough point to overcome drug-resistance of gastric-cancer problem. A great number of lncRNAs are expressed specific in the drug-resistance of gastric-cancer,using the traditional research methods that knockout and inhibit the lncRNA one by one consume a lot of time and resources, poor feasibility. The obstacle that the depth cooperation of bioinformatics and biology to solve this difficult problem is that the structural features of lncRNA and its biological functions are closely related and the key of lncRNA's function is the secondary structure, the prediction softwares of secondary structure are concerned about the accuracy of the global structure, ignoring the more important function components of the local conservation structure , and can not meet the length demand of lncRNA's structure function. Therefore, the project proposes a model of lncRNA structure prediction based on sliding window and component to guide the biological experiments of the drug-resistance of gastric-cancer, providing an effective way by using lncRNA to reverse and terminate the drug-resistance of gastric-cancer, it also provides a reference to overcome other drug-resistance of cancers.

英文关键词： Bioinformatics Processing;Gastric Cancer;MDR;lncRNA;RNA Secondary Structure