VvYABBY-5 基因调控葡萄多心皮子房形成分子机制研究

项目名称： VvYABBY-5 基因调控葡萄多心皮子房形成分子机制研究

项目编号： No.31471842

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 胡建芳

作者单位： 中国农业大学

项目金额： 85万元

中文摘要： 葡萄果实一般为2心皮4胚珠植物，但极少数品种在自然条件下果实可以发育成多心皮（3-4心皮）。多心皮比2心皮果粒大，果穗重，产量高，具有重要的生产价值，但目前有关多心皮报道极少，其形成机理尚不清晰，而研究多心皮发生机制能为大果粒育种及生理调控提供依据。本研究以多心皮发生率较高的葡萄香妃为试材，在前期研究基础上，首先利用realtime-PCR和原位杂交对心皮发生有重要作用的VvYABBY-5基因在不同心皮数和发育阶段的子房进行时空表达分析，明确VvYABBY-5基因表达与多心皮子房的相关关系；其次转基因增强和抑制VvYABBY-5基因表达，观察转基因植株心皮变化，确定VvYABBY-5基因调控心皮数目的作用；最后利用转录组测序结合染色质免疫共沉淀筛选鉴定被转录因子VvYABBY-5基因调控的下游功能基因，转基因研究其功能。最终揭示以VvYABBY-5基因为核心的调控葡萄多心皮发生的分子机制。

中文关键词： 基因克隆；转录因子；转录组测序；功能分析；种质资源

英文摘要： Grape fruit generally has two carpels which encloses two ovules respectively. However, a few cultivers produce fruits with three or four carpels in natural condition. Comparing to the two carpels, Fruit size, bunch weight and yield of multiple carpels are be improved. However, the mechanism of the increase in carpel number is still unknown and the study for this mechanism will offer the insights into physiologic regulation and breeding big fruit grape. In this work, we are ready to use xiangfei grape as the plant material because of the high three carpel percentage in this cultivar. Firstly, the spatiotemporal expression of the candidate gene VvYABBY-5 which controlling the carpel number during development of the ovaries with different number carpel (two, three and four carper) will be analyzed by Realtime-PCR and In situ hybridization. Secondly, the role of VvYABBY-5 in multiple carpels forming will be analyzed by overexpressing and silencing VvYABBY-5 in xiangfei grape and calculating multiple carpel percentage in transgenic grape. Finally, the functional genes those are the downstream targets of VvYABBY-5 will be found through transcriptome profiling and chromatin immunoprecipitation. Then the role of these genes will be analyzed by transgenic technology. The aim of this proposal is to reveal the he genetic networks in controlling the formation of multiple carpel.

英文关键词： Gene cloning;Transcription factor;Transcriptome sequencing;Functional analysis;Gene resources