新疆酿酒葡萄紫檀茋防御酸腐病的机制研究

项目名称： 新疆酿酒葡萄紫檀茋防御酸腐病的机制研究

项目编号： No.31460411

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 任雪艳

作者单位： 石河子大学

项目金额： 47万元

中文摘要： 酸腐病不但引起酿酒葡萄采后期烂果发生，而且还严重影响酿制葡萄酒品质。申请者项目组前期研究葡萄中紫檀茋对酸腐病防御呈显著性，且在药理浓度下紫檀茋体外高效抑制酸腐菌活性，但葡萄中紫檀茋对酸腐病防御机制尚不清楚，故本项目拟在前期研究工作基础上，首先使用扫描电镜、透射电镜、流式细胞、qPCR等技术进行酸腐菌体外抑菌机制研究；其次，以新疆酿酒葡萄为试验材料，酸腐菌侵染葡萄，分离紫檀茋防御形成中间产物，使用NMR / HPLC/ESI-MSn技术鉴定中间产物结构；然后使用深度测序（Deep sequence）/qPCR技术获得差异表达基因, 挖掘可能参与紫檀茋防御酸腐病关键基因，并分别在体外酵母、体内葡萄果实真核表达进行验证；最后在上述的研究基础上，根据体外抑菌机制，结合防御中间产物及其防御关键基因阐明酿酒葡萄紫檀茋防御酸腐病的机制。本项目研究结果有助于明确酿酒葡萄茋类化合物防御酸腐病机制。

中文关键词： 紫檀茋；酸腐病；新疆酿酒葡萄；防御机制

英文摘要： Grape quality may be affected by a wide range of sour rots. Among them, sour rot is an emergent grapevine disease affecting late ripening cultivars, causing heavy crop losses and being detrimental to wine quality. Our previous work showed that the pterostilbene defensed significantly in both dose-dependent and time-dependent manners to G.citri-aurantii inoculated grape berries, and it was very effective antifungal activities at pharmacological concentrations in vitro. However, it was not well know that the mechanism of pterostilbene defence to G.citri-aurantii in grape. On the base of our previous study, first, the mechanism action of pterostilbene in Xinjiang wine grape against the growth of G.citri-aurantii will be studied by transmission electron, scanning electron microscopy, flow cytometry and qPCR in vitro. Secondly, Xinjiang wine grape is used to study experimental material, which was inoculated with G.citri-aurantii, the structures of metabolic intermediate were elucidated during the course of pterostilbene defence by spectroscopic analysis, including HPLC/ESI-MSn and NMR experiments. Thirdly, differentially expressed gene were identified by using deep sequencing and qPCR. And which was combined with metabolic intermediate of pterostilbene defence to infer possible key gene involved in the process. We will verify the results by expressing these genes using yeast in vitro and grape berries in vivo, respectively. The relationship of metabolic intermediate and key genes of pterostilbene defence will be established. Finally, base on all these research results, the mechanism of pterostilbene defence to G.citri-aurantii in wine grape will be expounded. The results of this project will help us understand the mechanism of stilbenes defensing to G.citri-aurantii in grape.

英文关键词： Pterostilben;Sour rot;Xinjiang wine grape;Defence mechanism