基于微流控传感器阵列的细胞免疫及结核感染同步检测芯片的研究

项目名称： 基于微流控传感器阵列的细胞免疫及结核感染同步检测芯片的研究

项目编号： No.81472034

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 蒲晓允

作者单位： 中国人民解放军第三军医大学

项目金额： 65万元

中文摘要： 结核病是临床常见病，其发病率高、传染性强、危害大，结核杆菌感染是其直接原因，但其发病、转归还与机体细胞免疫功能、细胞因子分泌密切相关，因此联合检测细胞免疫功能和结核感染对临床治疗和预后判断至关重要。目前，虽可用流式细胞术和ELISA等分别检测，但由仪器贵、操作繁、通量低等缺陷。本项目将微流控、阵列传感、免疫捕获及适体技术有机结合，将不同T细胞亚群由抗体捕获于不同叉指传感器，并完成细胞计数，遂以结核特异抗原刺激捕获的淋巴细胞，使之释放γ-干扰素、肿瘤坏死因子-α和穿孔素，用适配体修饰的传感器分别原位检测三种因子，以实现细胞免疫功能、细胞因子与结核感染的同步检测，该芯片具有操作简、特异性强、通量高、速度快等优点,集多目标多功能检测于一体。本研究将为临床及早诊断结核感染、选择最适治疗方案、阐明结核的发生发展规律提供新的技术手段，将推动同类技术和研究更好、更快地应用和服务于临床。

中文关键词： 微流控芯片；生物传感器；结核感染；细胞免疫

英文摘要： Tuberculosis is a common disease, which has high incidence, strong contagious and great harmful. Although Mycobacterium tuberculosis is the main pathogens, immune function of body and cell factors are closely correlated with tuberculosis incidence and disease outcome. So combined detection of cellular immune function and Mycobacterium tuberculosis infection is essential for clinical treatment and prognosis. Nowadays the flow cytometry and ELISA are respectively available, but there are some defects, for instance, expensive instrument, complicated operation, low the flux. The project will use micro fluidic, array sensing, immune capture and aptamer technology. Capture the T cells on different interdigital transducer through the antibody binding and count number of T cell. Then stimulate lymphocytes captured with tuberculosis -specific antigen to release IFN-γ、TNF-α, and perforin, which will be detect in situ with aptamers sensors detect. It would be implement synchronous detection of cell immune, cytokines and tuberculosis infection. The chip has many merits, for example, simple operation, high flux, fast speed, multi-target detection in one multi-functional etc.. This research will provide a new method for an early clinical diagnosis of tuberculosis infection, selecting the optimal treatment regimen, clarifying the occurrence of tuberculosis. It would promote the progress better and faster of the similar technologies and researches for clinic medicine.

英文关键词： Microfluidic chip;Biosensor;Tuberculosis infection;Cellular immunity