根际效应对双加氧酶基因多样性、表达和蛋白的影响及与多环芳烃降解的关系

项目名称： 根际效应对双加氧酶基因多样性、表达和蛋白的影响及与多环芳烃降解的关系

项目编号： No.41271336

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 天文学、地球科学

项目作者： 巩宗强

作者单位： 中国科学院沈阳应用生态研究所

项目金额： 75万元

中文摘要： 针对多环芳烃原污染及模拟农田土壤，应用分室根箱实验进行植物-微生物联合修复，应用植物根系分泌物及其组分进行模拟根际修复，并建立根系分泌物-纯培养菌微宇宙，设计针对多种双加氧酶的兼并引物，用qPCR、RT-qPCR、分子克隆测序、双向电泳及飞行时间质谱等手段分析根际效应对微生物双加氧酶多样性、表达及相关蛋白特征的影响机理，探讨多环芳烃根际降解与微生物双加氧酶的关系。项目成果可阐明根系分泌物浓度、组分对农田土壤微生物多种双加氧酶基因多样性数量、组成及表达特征变化规律的影响机理；揭示根际效应作用下对土壤多环芳烃降解起重要作用的双加氧酶基因、蛋白及微生物种类；建立根际效应作用下多环芳烃降解与土壤微生物双加氧酶数量、组成和表达特征间的相关联系；深入认识污染土壤修复中的植物-微生物作用关系。本项目可以为利用根际效应促进多环芳烃的降解提供依据，为多环芳烃污染土壤修复理论与技术的进一步发展奠定基础。

中文关键词： 生物降解；植物修复；加氧酶；基因表达；根际效应

英文摘要： With polycyclic aromatic hydrocarbons (PAHs) contaminated field soil and spiked soil as target soils. This project will carry out integrated phyto-microbial remediation of contaminated soils through compartmented rhizo-box experiments, conduct simulated rhizodeposition remediation by the application of root exudates or their components, and set up microcosms consisting of root exudate and cultured strains. Degenerative primers will be designed, quantitative PCR, reverse transcript-PCR, molecular cloning-sequencing, two dimensional electrophoresis and time of flight mass spectrometry will be used to analyze the rhizospheric effects on the characteristics of dioxygenase gene abundance, diversity, expression and related proteomic profiling. The relationship between PAH degradation in rhizosphere and microbial dioxygenase will also be investigated in this study. The results of the project can help to 1) clarify the effects of root exudate concentration and components on characteristics of dioxygenase gene abundance, diversity, and expression of microorganisms in agricultural soil, 2) identify dioxygenase genes, proteins, and microbial populations which play an important role in degradation of PAH in soil under rhizosphere effects, 3) understand the relationships between PAH degradation and characteristics of soil mi

英文关键词： Biodegradation；Phytoremediation；dioxygenase；genes expression；rhizosphere effect