对低剂量氧化石墨烯致秀丽线虫寿命缩减毒性敏感的突变背景鉴定及形成机制研究

项目名称： 对低剂量氧化石墨烯致秀丽线虫寿命缩减毒性敏感的突变背景鉴定及形成机制研究

项目编号： No.81202233

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 预防医学、地方病学、职业病学、放射医学

项目作者： 武秋立

作者单位： 东南大学

项目金额： 23万元

中文摘要： 氧化石墨烯（GO）是在药物载体、生物传感器制造、生物成像等领域具有潜在应用价值的一种纳米材料，但针对低剂量GO暴露可能产生不利生物学效应及可能毒作用机理尚未进行系统与深入的解析。模式动物秀丽线虫已广泛应用于环境毒物毒性评价以及毒理学研究，其寿命评价终点可有效反映特定毒物可能长期毒性效应。为此本项目将分析低剂量GO暴露对于秀丽线虫寿命以及衰老形成相关性状的影响，解析低剂量GO暴露诱导特征性蛋白质表达谱以及差异表达蛋白对应基因可能调控衰老的功能，进而揭示对于低剂量GO暴露诱导寿命缩减毒性效应敏感的突变背景以及代表性敏感突变背景形成的内在分子机制。该项目将不仅可以解析低剂量GO暴露诱导毒性效应以及敏感突变背景形成相应内在分子机制，还将为低剂量GO暴露诱导长期毒性效应提出有价值评价终点。

中文关键词： 纳米毒理学；秀丽线虫；氧化石墨烯；敏感突变背景；分子机制

英文摘要： Graphene oxide (GO), due to its potential applications such as drug delivery, biosensor and bioimaging, has presently attracted much attentions; however, the adverse effects and the corresponding formation mechanisms for low doses of GO exposure are still unclear. Model organism of Canenorhabditis elegans has made many contributions to the study of environmental toxicity evaluation and toxicology, and the lifespan endpoint of C. elegans can reflect the potential long-term toxic impacts of specific toxicants. In this project, we will analyze the adverse effects of low doses of GO on lifespan and relative phenotypes, and analyze the characteristic protein expression profile and functions of differentially expressed proteins in lifespan control, and further identify the sensitive mutation backgrounds and their formation mechanism for toxicity on lifespan in C. elegans exposed to low doses of GO. Our study will elucidate the formation mechanisms of sensitive mutation background for toxicity on lifespan of nematodes exposed to low doses of GO, and present potential valuable endpoints for assessing the long-term toxicity of low doses of GO.

英文关键词： Nanotoxicology；Caenorhabditis elegans；Graphene oxide；Sensitive mutation；molecular mechanism