植物环蛋白的组装：一种新型天然超分子材料及其作为药物载体的研究

项目名称： 植物环蛋白的组装：一种新型天然超分子材料及其作为药物载体的研究

项目编号： No.21204086

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 高分子科学

项目作者： 许文彦

作者单位： 中国科学院长春应用化学研究所

项目金额： 25万元

中文摘要： 目前药物输送载体主要为人工合成的超分子材料，虽然有其自身优势，但疏水内腔限制了对亲水分子的输送。近年报道的病毒衣壳蛋白虽可以输送几乎所有的药物分子，但单体分子量过大，代谢过程复杂，毒性不明等问题同样制约其应用。 植物环蛋白是一类具有特殊结构且非常稳定的天然多肽分子。其首尾环合的骨架及半胱氨酸结使分子具有类似小分子的刚性，且对水解酶、酸、有机溶剂和高温均具有较高抗性。部分较长的环结构又使分子具有类似大分子的柔性。根据疏水性残基与亲水性残基分别集中分布于分子不同部位的特征，申请者推测环蛋白分子具有良好的自组装潜力，并得到前期研究结果的支持。 本项目旨在研究植物环蛋白的自组装行为及其超分子自组装体的性质。通过系统地研究环蛋白浓度、组装环境和组装条件对组装过程及超分子结构的影响，寻找合适的条件组合使植物环蛋白超分子自组装体具有药物输送的功能。

中文关键词： 环蛋白；乳液；药物输送；；

英文摘要： A lot of superparticles have been synthesized or discovered as carriers of medicinal molecules in recent years, such as cyclodextrins and caspids of virus. Although these nanomaterials show some good characters, the disadvatages are also obvious. For example, cyclodextrins prefer to load hydrophobic molecules; and caspids show potential risk to the human health, which blocks the way to use them in clinic. Cyclotide is a fantastic family of natural peptide, which presents a stable and special structure. The head-to-tail cyclic backbone and cyclic cystine knot make the structure as rigid as small molecules,and also make cyclotide resistant to acid, organic solvent, heat and enzymatic hydrolysis. Some long loops make cyclotide as flexible as macromolecules also. Hydrophobic and hydrophobic residues present in different areas of the structure, which indicates that cyclotide could assemble to be superparticle. This project is going to study the self-assembly of cyclotide and the characters of the superparticle. The research on relationships between concentration of cyclotide and surrounding enviroment (such as ions, pH, and temperature) to the assembling process of cyclotide superparticle would be helpful to build up a novel carrier for pharmaceutic compounds.

英文关键词： cyclotide；Emulsion；Drug Delivery；；