基于灰色关联分析方法对鹿茸“补肾健骨”作用与品质相关性研究

项目名称： 基于灰色关联分析方法对鹿茸“补肾健骨”作用与品质相关性研究

项目编号： No.81473314

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李峰

作者单位： 辽宁中医药大学

项目金额： 73万元

中文摘要： 针对鹿茸功效与品质相关性研究的空白，本研究提出基于灰色关联度分析方法对鹿茸补肾健骨作用与品质相关性进行研究。拟选择具代表性的不同品质的鹿茸药材为研究对象，通过其对小鼠成骨细胞增殖与分化、大鼠骨髓间充质干细胞增殖及向成骨分化、以及对去卵巢大鼠所致骨质疏松防治作用研究，以灰色关联度分析方法的相对关联度ri，建立鹿茸补肾健骨作用与品质的相关性及关联程度；同时，测定不同品质的鹿茸中的各主成分含量，并分别以此为自变量、鹿茸补肾健骨的综合效应指数为因变量，进行逐步回归分析，确定对鹿茸补肾健骨作用影响最显著的化学成分或成分类群，即化学表征（含量测定指标）；在此基础上，对放大鹿茸药材的样本，进行表征成分含量测定与聚类分析，将得到的各类鹿茸药材性状特征进行描述，即性状表征（性状鉴别指标）。以期建立以临床功效为核心与导向的新型鹿茸及中药材品质评价模式与指标体系。

中文关键词： 品质评价；灰色关联分析；鹿茸；补肾健骨；药效与品质相关性

英文摘要： Nowadays, due to the lack of research on the correlation between CERVI CORNU PANTOTRICHU effect and its quality, a new method, based on the grey correlation analysis, is put forward for the first time to study the correlation between the CERVI CORNU PANTOTRICHU quality and its effect of tonifying the kidney and strengthening the bone. The research can be divided into three sections. Firstly, different qualities of CERVI CORNU PANTOTRICHU are chosen. The effects of CERVI CORNU PANTOTRICHU on the proliferation and differentiation of both MC3T3-E1 and BMSCs are studied. Also the effect of CERVI CORNU PANTOTRICHU on ovariectomized rats as a pharmacological model of osteoporosis is studied. Secondly, calculate the relative correlation degree (ri) with the grey correlation analysis.The correlation between CERVI CORNU PANTOTRICHU effect and its quality is established according to the result. The main compositions from different qualities of CERVI CORNU PANTOTRICHU have been determined at the same time. Then, Stepwise regression analysis is used the main compositions content as the variables and thecomposite effect indexas the dependent variables. The compositions which have the greatest affect upon tonifying the kidney and strengthening the bone are determined as the chemical characteristic. Finally,the samples of CERVI CORNU PANTOTRICHU are added, and the characteristic compositions are determined and cluster analysis is processed. Macroscopic identification of the sub-classification are described as the macroscopic characteristic. A new quality evaluation pattern and indicator system about CERVI CORNU PANTOTRICHU （Chinese crude drugs）can be established on the base of its clinical effect.

英文关键词： quality evaluation;grey correlation analysis;CERVI CORNU PANTOTRICHUM;tonifying the kidney and strengthening the bone;correlation between efficacy and quality