针对F-和CN-离子的水溶性双光子荧光探针的设计与合成

项目名称： 针对F-和CN-离子的水溶性双光子荧光探针的设计与合成

项目编号： No.20802026

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 曹笃霞

作者单位： 济南大学

项目金额： 18万元

中文摘要： 荧光探针在生物体系离子/分子检测方面具有重要应用。本项目努力将当前荧光探针和双光子荧光两方面的研究前沿结合起来，利用双光子荧光性质作为检测信号手段，设计新的针对于氟离子及氰根离子的荧光探针。 本课题主要是利用有机硼化合物是天然的强Lewis酸，可与氟离子、氰根离子等典型Lewis碱发生作用的特点，设计合成具有双光子荧光的水溶性有机硼化合物并实现对氟离子和氰根离子进行高选择性高灵敏性的检测。完成的研究内容主要有以下几方面：以二米基硼和硼酸为氟离子和氰根离子结合位点，采用严格无氧无水反应条件，设计合成出多种新的稳定的有机硼化合物。系统研究了化合物的单双光子吸收和荧光性质，总结了分子结构与性质之间的关系。系统研究了化合物对氟离子和氰根离子的单双光子荧光识别情况。设计并合成出一批羟基查尔酮和酰胺化合物，系统研究了化合物对氰根离子的识别情况，实现了对氰根离子单双光子荧光turn on型识别。对识别机理进行了分析与探讨。

中文关键词： 双光子荧光；水溶性；荧光探针；有机硼化合物

英文摘要： Fluorescence sensors exhibit important application in detecting biologic ions or molecules. This research project combine the two frontier researches of fluorescence sensor and two-photon excited fluorescence. The main tasks are design and synthesis of fluorescence sensor to fluoride and cyanide anions with two-photon fluorescence as the detecting signal. Organoboron compounds are crude strong Lewis acid and can react with typical Lewis alkali such as fluoride and cyanide anions. This research project is devoted to the design and synthesis of water soluble organoboron compounds with two-photon fluorescence, which can recognize fluoride and cyanide with high selectivity and sensitivity. The main research contens are as following. Many new stable organoboron compounds were synthesized by strict oxygen and water free. Their single- and two-photon excited fluorescence properties are systematically investigated and the relationships between the structure and property are summarized. The recognization of the compounds to fluoride and cyanide anions are systematically investigated. Several series of hydroxy chalcone and coumarin amide derivatives are synthesized and their recognization to cyanide anions are systematically investigated. These compounds can recognize cyanide with turn-on single- and two-photon excited fluorescence. The binding mechanisms of these compounds with cyanide anions are also analyzed and investigated

英文关键词： two-photon fluorescence; water solubility; fluorescent chemosensor; organoboron compound