项目名称: 脂肪酸调控皱纹盘鲍Δ5脂肪酸去饱和酶基因表达的分子机制
项目编号: No.31502173
项目类型: 青年科学基金项目
立项/批准年度: 2016
项目学科: 农业科学
项目作者: 李明珠
作者单位: 鲁东大学
项目金额: 19万元
中文摘要: 脂肪酸去饱和酶(Fad)是动物体利用C18多不饱和脂肪酸(C18PUFA)合成长链多不饱和脂肪酸(LCPUFA)的关键酶。脂肪酸调控Fad基因表达的分子机制仍不清晰。本项目以皱纹盘鲍为研究对象,运用现代营养学、生物化学和分子生物学的方法,研究了转录因子胆固醇调控元件结合蛋白-1(SREBP-1)和过氧化物酶体增殖物激活受体α(PPARα)对Δ5Fad基因启动子的调节作用,探讨了脂肪酸对Δ5Fad、SREBP-1和PPARα基因表达的影响,阐明了脂肪酸影响Δ5Fad基因表达受SREBP-1和PPARα调控的分子机制。通过该项目的研究,一方面填补了贝类在该领域的研究空白;另一方面为提高鲍鱼利用植物油中的C18PUFA来合成LCPUFA的能力提供理论依据。
中文关键词: 代谢;脂肪酸去饱和酶;基因表达;长链多不饱和脂肪酸;鲍鱼
英文摘要: Fatty acyl desaturase (Fad) is the crucial enzyme for biosynthesis of long chain polyunsaturated fatty acid (LCPUFA) from C18 polyunsaturated fatty acid (PUFA). The molecular mechanism of dietary fatty acid regulate the gene expression of fatty acyl desaturase (Fad) in aquatic animal is still not clear. Using the technologies of modern nutriology, biochemistry and molecular biology, this project will be conducted to study the regulation of sterol regulatory element binding proteins 1 (SREBP-1) and peroxisome proliferator-activated receptors α (PPARα) on the Δ5 Fad promoter of abalone (Haliotis discus hannai Ino), investigate the effect of dietary fatty acids on gene expression of Δ5 Fad, SREBP-1 and PPARα and consequently illuminate the involvement of SREBP-1 and PPARα in the regulation of abalone Δ5 Fad by dietary fatty acid. This study not only fill the gap in this research field but also provide theoretical basis for improving the capability of abalone to biosynthesize LCPUFA from C18 precursors.
英文关键词: Metabolism;Fatty acyl desaturase;Gene expression;Long chain polyunsaturated fatty acid;Abalone