三七生长发育和皂苷积累对低温的响应及其调控机制研究

项目名称： 三七生长发育和皂苷积累对低温的响应及其调控机制研究

项目编号： No.81460581

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 徐福荣

作者单位： 云南中医药大学

项目金额： 45万元

中文摘要： 三七［Panax notoginseng (Burk)F.H.Chen］是五加科人参属多年生药用植物，是我国特有、传统名贵中药材，是云药产业发展的第一大支柱产业。由于三七皂苷独特的药理、药效，致使需求量剧增，同时受连作障碍影响，道地主产区文山州土地资源紧缺，现逐渐向高纬度、高海拔地区异地引种，低温已成为影响三七生长发育及其皂苷积累的一个主要因素。本研究采用中药资源生态学和分子生物学方法，通过自然和人工控温处理，揭示三七的生物学、光合生理、脂质过氧化及保护酶活性低温响应状况；利用高效液相色谱(HPLC)与近红外光谱双指标序列分析三七化学特征组/成分的变化差异，阐明低温对三七皂苷积累及其质量的影响。利用抑制消减杂交(suppression subtractive hybridization,SSH)技术构建三七低温胁迫差异表达基因cDNA文库，解析低温诱导三七皂苷积累相关基因及其调控机制。

中文关键词： 三七；低温；调控机制；生长发育；皂苷积累

英文摘要： Panax notoginseng (Burk)F. H. Chen, which belongs to the genus Panax in the Aralisceae, is one of the most well-known and valued Chinese medical herbs. It has been also designated as the first-place pillar industry for the development of the Yunnan pharmaceutical industry in Yunnan Province. Wenshan is the main genuine production area of Sanqi. In recent years, people began to expand Sanqi cultivation in other areas of Yunnan, as fewer lands in Wenshan are suitable to cultivate it due to the obstacle of continuous cropping. Owing to its special pharmacological effect, Sanqi production has increased dramatically in recent years. As its cultivation moved to the higher latitude and altitude, low-temperature has become one of the important factors influencing the growth and saponins accumulation. In this research, various methods such as molecular technique and ecological tools for traditional Chinese medicine resources will be applied. The impacts of the low-temperature on the biological characteristics photosynthetic physiology, lipid peroxidation and protecting enzymatic activity will be evaluated under natural and controlled conditions of low temperature. The relationships between the low-temperature and the chemical constituents from sanqi will be assessed using the two analytic methods: High Performance Liquid Chromatography (HPLC) and near infrared (NIR) spectroscopic methods, with the hope of evaluating how the low temperature affects the saponin accumulation and its quality. The cDNA Library of the differentially expressed genes from Sanqi under low-temperature stress will be developed using the SSH technology, and the regulatory mechanism and the genes relating to the accumulation of the notoginsenosides will be analyzed. The research outcome will help understand the adaptability of Sanqi to low temperature and provide the baseline information for a healthy development of the sanqi industry.

英文关键词： Panax notoginseng;low temperature;regulatory mechanism;growth and development;saponin accumulation