一种基于TGA/TGH共修饰酸度敏感型肿瘤双靶向纳米探针的研制与宫颈癌早期诊断研究

项目名称： 一种基于TGA/TGH共修饰酸度敏感型肿瘤双靶向纳米探针的研制与宫颈癌早期诊断研究

项目编号： No.81201696

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学1

项目作者： 杨冬芝

作者单位： 徐州医学院

项目金额： 23万元

中文摘要： 纳米量子点具有优良的光谱性质，但其纳米粒径和丰富的表面电荷决定了它在细胞表面的强烈吸附和被细胞吞噬的可能，因而量子点未修饰前无法完成对正常细胞和肿瘤细胞的特异性识别。改善表面性质、增加特异性基团是实现量子点对肿瘤细胞定向识别的有效策略。申请者前期研究发现，利用酸度敏感的酰腙键作为PEG与量子点的连接键，可实现量子点对于特定酸度环境的识别。基于肿瘤细胞周围环境的特殊性，本项目拟以制备TGA/TGH共修饰的酸度敏感型纳米量子点为切入点，将宫颈癌肿瘤标志物与其相链接，构建酸度敏感/肿瘤标志物双靶向纳米探针；应用于培养细胞和动物模型，在细胞和分子水平研究，实现对宫颈癌的早期诊断。预期结果:实现酸度敏感型纳米探针的构建；完成对肿瘤细胞的特异性识别；通过动物模型实现在体宫颈癌的早期诊断、荧光成像及准确定位。本课题将为宫颈癌早期诊断提供双靶向、智能化的检测新方法，也为纳米探针用于肿瘤早期诊断提供新思路。

中文关键词： 量子点；酸度敏感型；双靶向；生物安全性；细胞成像

英文摘要： Quantum dots (QDs) have excellent optical characteristics, but they are easy to be adsorbed or swallowed by cells because of their nano-particle size and so many surface charges. So, they can not achieve the specific recognition of normal and tumor cells. Changing their surface properties or increasing specific groups is one of effective strategies. In my preliminary study, QDs connected PEG through acidity-sensitive hydrazone bond can identify some particular acidity. Based on the special environment of tumor cells, this project intends to start with preparation pH-sensitive TGA/TGH modified QDs, and then links the tumor markers of cervical cancer to QDs, to build the dual targeting nano-probe. Used on cultured cells and animal models, this nano-probe would achieve early diagnosis and accurate localization of cevival cancer on the cellular and molecular levels. This study expects to complete the preparation of pH sensitive nano-probes. This probe would identify tumor cells from normal cells and achieve early and specific diagnosis of cervical cancer cells. Furthermore, it would achieve the early diagnosis, fluorescence imaging and and tracing of cervical cancer in vivo of animal models. This work will not only give a new dual targeting and intelligent method for the early diagnosis of cervical cancer, but also

英文关键词： quantum dots；pH sensitive；dual targeting；bio-security；cells labeling