利用RNA-Seq技术分离鉴定柳枝稷分蘖发育调控相关基因

项目名称： 利用RNA-Seq技术分离鉴定柳枝稷分蘖发育调控相关基因

项目编号： No.31301384

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 孙风丽

作者单位： 西北农林科技大学

项目金额： 22万元

中文摘要： 分蘖是禾本科植物分枝的特化形式，分蘖数是禾本科作物的重要农艺性状之一，有效分蘖数直接影响作物的产量，因此研究分蘖的调控机理对农业生产具有重要理论意义。当前有关分蘖的研究主要在水稻中，一般通过突变体克隆相关基因，对分蘖发育的调控机理的认知还有待完善。生物能源植物柳枝稷具有高效的分蘖能力，是研究禾本科植物分蘖的理想材料。本项目组已经通过EMS 诱变创制了丧失高效分蘖能力的柳枝稷突变材料。拟采用高通量RNA-Seq 技术分别对分蘖盛期的突变型和野生型材料的茎尖生长点进行转录组分析，筛选差异表达基因，利用DNA 末端快速扩增技术（RACE）克隆候选基因的全长cDNA，通过基因表达谱分析、转基因超量表达和抑制表达（RNAi）进行候选基因的功能验证，揭示柳枝稷分蘖大量产生的分子机理，探索高等植物分枝发育的调控机理，为高等植物分枝发育的调控理论体系提供新数据，为禾本科作物的育种提供理论依据和基因资源。

中文关键词： 柳枝稷；分蘖；植物激素；转录组测序；基因克隆

英文摘要： Tiller is a specialized grain-bearing branch inmonocotyledonous plants. Tillering is one of the most important agronomic traits that determine grain yields. So researching on molecular regulatory mechanisms of tillering make good sense for agriculture. To this day, the research on tillering was mainly carried out in rice. And there were two ways to research on tillering: separating genes by mutant with unnormal tillering and mapping quantitative trait loci（QTL） associated with tillering. There are some important genes had been separated by studying on mutant but no any gene by QTL analysis. However the molecular mechanism of tillering still remains to be elucidated. In order to kown the generally regulatory mechanisms of tillering in monocotyledonous plants, more researchment on tillering in other species and efficient techniques were necessary. Switchgrass (Panicum virgatum L.), an important biofuel crop, were developed into perfect material for studying on tillering because of its high tillering ability. The mutant of Switchgrass with few tillers had been developed in our laboratory by EMS. In this proposal, we plan to sequence the shoot RNA of mutant and wild type in tillering period separately. Sequencing of RNA has long been recognized as an efficient method for gene discovery and remains the gold standard

英文关键词： switcgrass；tillering；plant hormones；RNA-Seq；gene cloning