红景天苷对膳食中潜在致癌物呋喃所致基因毒性的干预机制研究

项目名称： 红景天苷对膳食中潜在致癌物呋喃所致基因毒性的干预机制研究

项目编号： No.31471666

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 闫海洋

作者单位： 吉林大学

项目金额： 79万元

中文摘要： 呋喃（Furan）是一种重要的食品内源污染物，具有肝毒性和潜在的致癌性，国际癌症研究机构（IRAC）将呋喃归类为可能使人类致癌的物质2B组。呋喃的基因毒性是目前研究的热点和难点之一，但是，现有研究结果并未从基因变化和靶基因角度阐明呋喃毒作用机制。因此，本项目在前期研究的基础上，旨在借助全基因组芯片表达谱技术、荧光定量PCR和Western-blot等相关分子生物学手段，从实验动物水平对呋喃致基因毒性的靶基因进行筛选，从基因、分子及蛋白水平分析并筛选呋喃造成实验动物基因损伤的靶基因，明确呋喃致基因毒性机制；并利用红景天苷强效的呋喃毒性保护作用，通过实验动物中相关组织特征指标、DNA加合物含量、靶基因以及微观结构变化等，从DNA、基因和蛋白水平等方面阐明红景天苷对呋喃所致基因毒性的干预机制，以此指导减少呋喃对人体危害的新途径，最终为红景天苷在呋喃危害防护领域发挥的突出作用提供理论依据。

中文关键词： 呋喃；红景天苷；基因芯片；靶基因；干预机制

英文摘要： Furan is an important endogenous contaminant in food, which has liver toxicity and potential carcinogenicity. Furan has been classified by International Agency for Research on Cancer (IRAC) as possibly carcinogenic substance to humans (Group 2B).Genotoxicity of furan is one of the hot spots of the present study. But the researches of most scientists obtained recently were still lack of overall screening the target genes of furan-induced genotoxicity based on the target genes. On the basis of the preliminary studies, the project aims to screen the target genes of furan-induced genotoxicity based on the whole genome microarray expression technology, real time RT-PCR and Western-blot technology and others molecular biology methods from the level of experimental animals. We strive to explore the genetic damage mechanism of furan from DNA molecule, genes, and protein aspects. Meanwhile, in our previous study, we found that salidroside showed excellent protective effect against furan-induced liver toxicity. Salidroside will be added to study the protective mechanisms of furan-induced genotoxicity from the changes of DNA adducts and target genes. The protective mechanisms of salidroside against furan-induced genotoxicity are evaluated from DNA molecule, genes, and protein aspects. These results lay the foundation to further develop and utilize

英文关键词： Furan;Salidroside;Gene chip;Target gene;intervention mechanism