多效性QTLs Ghd8和Ghd7.1调控的水稻穗发育和株型建成的基因网络解析

项目名称： 多效性QTLs Ghd8和Ghd7.1调控的水稻穗发育和株型建成的基因网络解析

项目编号： No.91335201

项目类型： 重大研究计划

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 邢永忠

作者单位： 华中农业大学

项目金额： 300万元

中文摘要： 水稻产量维系国家粮食安全。我们新近克隆了同时影响每穗粒数，株高和开花期的多效性QTLs Ghd7.1和 Ghd8，发现它们在遗传学上的互作显著改善株型。本研究，我们将以Ghd7.1和 Ghd8的近等基因系，转基因单株，突变体和二基因聚合单株为材料，从转录调控，蛋白互作等层面解析它们的互作机理。以幼穗，茎秆，叶枕和分蘖芽为样品，构建酵母双杂交筛选文库，通过酵母双杂交，染色质免疫共沉淀结合测序技术，以及DNase I 超敏感位点测序等分子生物学手段，分离与Ghd8及Ghd7.1互作的蛋白质；通过比较分析细胞分裂素，生长素等植物激素的合成途径和信号传导途径的变化，探索激素在Ghd7.1及Ghd8功能发挥过程中的作用。对350份资源种质材料进行Ghd7.1和Ghd8深度测序，在二位点水平上分析不同基因组合的效应和生态区分布特点。最终建立穗发育和株型建成的遗传调控网络，为水稻高产育种提供理论支撑。

中文关键词： 调控网络；穗发育；蛋白互作；穗形；株高

英文摘要： Rice grain yield is a key factor affecting national food security. Recently we have cloned the pleiotropic QTLs Ghd7.1 and Ghd8 with effects on spikelets per panicle, plant height and heading date, and found their genetic interaction effects on plant architecture. In this project, we will use the near isogenic lines of Ghd7.1 and Ghd8, their transgenic plants, mutants and the plants pyramiding both genes to elucidate the molecular mechanisms underlying panicle development and plant architecture. We will clarify their molecular basis of their interaction at the levels of transcription and proteins. We will construct yeast two-hybrid libraries each with young panicle, stem, leaf cushion and tillering buds as samples. We will isolate the proteins interacted with Ghd7 and Ghd8 by using the biological techniques of yeast 2-hybrid, chromatin immunoprecipitation followed by sequencing, DNase I hypersensitive site sequencing. We will explore the relationship between phytohormone and functional action of Ghd7 and Ghd8 by comparison of changing in pytohormone synthesis pathway or signal transduction pathway. Finally we expect to establish the gene regulatory network for panicle development and plant architecture. In addition, we will deep sequence Ghd7.1 and Ghd8 for a germplasm collection of 350 accessions, and analyze t

英文关键词： Regulation network；Panicle Development；Protein Interaction；Panicle Architecture；Plant Height